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Microbial DNA qPCR Multi-Assay Kits

For real-time PCR-based, application-specific microbial identification or profiling; includes 3–6 assay tubes in one box for 20 reactions
  • Complete kit for microbial profiling with assays, controls, and reagents 
  • Includes a panel of experimentally verified Microbial DNA qPCR Assays
  • Uses 5' hydrolysis probe detection for high sensitivity and specificity
Microbial DNA qPCR Multi-Assay Kits are a complete solution for real-time PCR-based microbial detection. Microbial DNA qPCR Multi-Assay Kits contain a panel of Microbial DNA qPCR Assays, which are a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enable quantitative real-time PCR. The kit also contains everything required for a successful qPCR run, including Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water and Microbial qPCR Mastermix.

What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).
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Microbial DNA qPCR Multi-Assay Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Lower limit of quantification (LLOQ)
The LLOQ is the lowest concentration of template that still falls into the linear range of the standard curve (see figure, Limit of detection versus lower limit of quantification). Across all Microbial DNA qPCR Assays, 93% have an LLOQ of <100 gene copies (see figure, The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity). 92% of microbial identification assays meet this LLOQ, as do 95% of virulence factor gene assays and 97% of antibiotic resistance gene assays (see figures, The LLOQ for microbial identification Microbial DNA qPCR Assays reveals high sensitivityThe LLOQ for virulence factor genes Microbial DNA qPCR Assays reveals high sensitivity, and The LLOQ for antibiotic resistance genes Microbial DNA qPCR Assays reveals high sensitivity).

Each Microbial DNA qPCR Assay is stringently tested to ensure that it detects only one target species or gene (see figure, Microbial DNA qPCR Assays are highly specific). For assays that do detect more than one target, a list of detected targets and in silico predictions is included on the product sheet.

This specificity is maintained even when samples have high species complexity, such as in stool, sputum, and plaque (see figure, Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples).
Microbial DNA qPCR Assays are designed to detect bacterial 16S rRNA gene and fungal ribosomal rRNA gene sequences for species identification, as well as detecting virulence factor genes and antibiotic resistance genes using PCR amplification primers and hydrolysis-probe detection.
The procedure for Microbial DNA qPCR Multi-Assay Kits is simple, and can be performed in any laboratory with a real-time PCR instrument. DNA is isolated from the sample using the appropriate QIAamp kit, and PCR reactions are set up using the appropriate Microbial qPCR Mastermix for the PCR instrument. Three control reactions are prepared for each sample, including Positive PCR Control, No Template Control, and the Microbial DNA Positive Control, as well as the Microbial DNA qPCR Assays. Real-time PCR is performed and data is analyzed using web-based data analysis software or Excel templates.
Microbial DNA qPCR Multi-Assay Kits are highly suited for the detection of related bacterial or fungal species, or microbial genes for antibiotic resistance or virulence factors, from a variety of samples.

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Brochures & Guides (2)
Microbial product configurator guide
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For application-specific microbial identification and profiling by real-time PCR
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FAQs (19)
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Kit Handbooks (1)
New version – For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
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Neisseria meningitidis Pathogenicity Microbial DNA qPCR Multi-Assay Kit

The Neisseria meningitidis Pathogenicity Microbial qPCR DNA Multi-Assay Kit is a research tool used for the detection of virulence factors from N. meningitidis. This multi-assay kit provides microbiology researchers with a convenient way to quickly detect virulence factor genes from N. meningitidis in isolated bacterial colonies, metagenomic samples, or other sample types. Virulence factors facilitate development of disease from infectious bacteria by allowing a microorganism to establish itself on the host, in part by subverting or eluding host defenses, allowing attachment to the host, production of toxins, etc. The kit contains assays for detecting N. meningitidis itself and the virulence factor genes lbpA and pilG. The assays use PCR amplification primers and hydrolysis-probe detection, increasing their specificity. The kit also contains Microbial qPCR Mastermix, Microbial DNA-Free Water, Microbial DNA Positive Control, and Positive PCR Control. The Positive PCR Control assay is included to test for the presence of PCR inhibitors and the efficiency of the polymerase chain reaction. Identification of whether pathogenic Neisseria meningitidis is present in a sample and what virulence factors may be involved in its pathogenicity is accomplished using data analysis software with imported CT values generated by the PCR instrument software. The simplicity of the product format and operating procedure allow routine and reliable screening of pathogenic Neisseria meningitidis from samples in any research laboratory with access to a real-time PCR instrument.

Gene Resource List
Name Microbial DNA Multi-Assay Kit Neisseria meningitidis Pathogenicity (BBID-1516Z-4)
Name Neisseria meningitidis
Associated Species Neisseria gonorrhoeae, Neisseria cinerea, Neisseria meningitidis
Accession NC_008767.1
Sensitivity (gene copies) 300
Green Gene ID 187019
Description Positive PCR Control Assay
Name PPC
Accession MB_PPC
Description lactoferrin-binding protein A
Name lbpA
Associated Species Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria lactamica
Accession VFG0264
Sensitivity (gene copies) 100
Virulence Factor Database ID VFG0264
Description pilus assembly protein PilG
Name pilG
Associated Species Neisseria meningitidis
Accession VFG0235
Sensitivity (gene copies) 30
Virulence Factor Database ID VFG0235

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