The QIAprep& Viral RNA UM Kit combines a liquid-based sample preparation step completed in only two minutes with real-time RT-PCR detection in a streamlined workflow. Users can automate this method with standard lab equipment for any throughput, assay and reaction need from single to multiplex testing.
The kit is compatible with dual-labeled probes, e.g., TaqMan® probes in multiplex one-step RT-PCR detection of one or more targets (altogether, up to 4 assays including the internal controls). The kit has been optimized for use with most real-time cyclers. A novel two-phase hot-start procedure ensures high specificity and sensitivity in real-time RT-PCR. For high in-process safety during virus detection, each kit contains reagents for the simultaneous detection of user-defined targets and two internal controls for confident result interpretation.
The first is the inhibition control that consists of a synthetic RNA with a unique and artificial sequence and its detection assay that can optionally be used to monitor successful amplification. The RNA IC is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. The second control is a sampling control, an assay that targets two human transcripts and can optionally be used to confirm that the primary sample tube contains human material and that RNA materials have not been degraded. Together, this allows correct interpretation of negative detection results.
|Assay ||Target(s) ||Reporter dye/color channel* ||Supply |
|Inhibition Control ||Synthetic IVT ||Cy5/Red ||Included in the kit, optional |
|Sampling Control ||Human B2M and RNase P genes ||HEX™/Yellow (two targets detected in the same channel) ||Included in the kit, optional |
|Passive reference dye ||- ||ROX/Orange ||Included in the kit, optional |
|Your viral RNA assay ||User-defined || |
Recommended: target detection in green, blue or NIR channel
|Provided by the user |
*Altogether, up to 4 assays or targets, including the internal controls and excluding the reference dye