Cat. No. / ID: 330171
Custom RT2 Profiler PCR Arrays are reliable tools for studying and verifying gene expression biomarkers in large numbers of biological samples. Whether the biomarkers are from a proprietary collection, literature research or DNA microarray analysis, QIAGEN’s expertise in real-time PCR detection provides quality, flexibility and consistency in biomarker validation experiments. QIAGEN’s genomewide coverage provides primers for over 100,000 genes covering the human, mouse, rat, Rhesus macaque, drosophila and dog genomes.
SYBR Green-based real-time PCR is a powerful technology for studying and validating gene expression biomarkers in large numbers of biological samples. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays. The high-quality primer design and master mix formulation enable the PCR array to amplify gene-specific products simultaneously under uniform cycling conditions.
Rigorous and robust quality assurance (QA) and quality control (QC) procedures covering primer design and quality, specificity and amplification efficiency provide peace of mind.
This combination provides Custom RT2 Profiler PCR Arrays with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.
Custom RT2 Profiler PCR Arrays are available in the following plate layouts (see the tables below and figure " Available plate layouts". Select the genes and the plate format required.
|Format||Number of plates (minimum)|
|8 genes, 12 samples per plate||12|
|12 genes, 8 samples per plate||12|
|16 genes, 6 samples per plate||12|
|24 genes, 4 samples per plate||24|
|32 genes, 3 samples per plate||24|
|48 genes, 2 samples per plate||24|
|96 genes, 1 sample per plate||24|
|All formats||Per additional 12 array|
|Format||Number of plates (minimum)|
|8 genes, 48 samples per plate||6|
|12 genes, 32 samples per plate||6|
|24 genes, 16 samples per plate||6|
|32 genes, 12 samples per plate||6|
|48 genes, 8 samples per plate||6|
|64 genes, 6 samples per plate||6|
|96 genes, 4 samples per plate||6|
|128 genes, 3 samples per plate||6|
|192 genes, 2 samples per plate||24|
|384 genes, 1 sample per plate||24|
|All formats||Per additional 6 array|
Arrays are available in additional formats or with special assay designs (position, variants, etc.), please inquire.
Simply mix the cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program (see flowchart " Simple procedure"). Custom RT2 Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche and Stratagene instruments.
Custom RT2 Profiler PCR Arrays are available in 96-well plate, 384-well plate and 100-well disc formats, and are used to monitor the expression of up to 84 or 370 genes related to a specific research area of interest, plus 5 housekeeping genes. Custom RT2 Profiler PCR Arrays may also include control elements for:
Data can be analyzed using an easy-to-use Excel-based data analysis template or web-based software. Data analysis is based on the ΔΔCT method with normalization of the raw data to either housekeeping genes.
Custom or Modified RT2 PCR Profiler Arrays offer a robust platform for biomarker screening and validation and can be used in all areas of biological and medical research, including:
Different amounts of universal total RNA were characterized using the Human Inflammatory Cytokines and Receptors RT2 Profiler PCR Array and the percentage of detectable genes calculated for each amount. As little as 25 ng total RNA yields an >80% positive call, even for cytokines expressed at very low levels.