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QIAamplifier 96

For fast and high performance end-point PCR experiments in a 96-well format
  • Fast cycling protocol, down to 45 minutes for 30 cycles
  • Temperature ramping rates up to 4°C/s
  • Temperature uniformity down to ±0.2°C across the sample block
  • Linear gradient function for assay optimization

The QIAamplifier 96 is a fast and high-performance 96-well block system for all end-point PCR requirements. It is equipped with a heated smart lid for consistency of results. Low noise with a maximum of 45 decibels when running makes it a low-profile lab companion. The QIAamplifier 96 is designed to last and will deliver consistent and reproducible results run after run.

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Cat No./ID: 9002990
QIAamplifier 96 (115 V)

Fast cycling, high performance thermocycler with a 96-well block and a color touchscreen interface for end-point PCR requirements. 115 V power specification.

Cat No./ID: 9002991
QIAamplifier 96 (230 V)

Fast cycling, high performance thermocycler with a 96-well block and a color touchscreen interface for end-point PCR requirements. 230 V power specification.

The QIAamplifier 96 is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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Comparison of amplification of a difficult locus TH01.

Comparison of amplification of difficult locus TH01 between QIAamplifier 96 and Applied Biosystems ABI 9700 showing overall equal profile peak heights and quality.
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Multiplex co-amplification and detection of 8 targets.

Multiplex co-amplification and detection of 8 targets ranging from 123 bp to 610 bp using QIAGEN UCP Multiplex PCR Kit. A Average concentration and standard deviation of the 8 targets. B Normalized concentration of targets with standard deviation. C Gel views of samples rows A, B and C using the QIAxcel DNA High Resolution Kit on the QIAxcel Advanced capillary electrophoresis instrument.
Performance

The QIAamplifier 96 is a high-performance thermal cycler with a 96-well block and a gradient function ideal for all your end-point PCR requirements. High expectations for long-term reliability and robustness are met with the well-thought-out design and assembly.

Principle

The QIAamplifier 96 is a stand-alone end-point PCR cycler with a 7-inch color touchscreen and an easy-to-use software interface. Users can choose between different programming modes and templates and a quick-start list.

Fast ramping, highest accuracy block control

The QIAamplifier 96 controls the sample block temperature without under- or overshooting the programmed target temperature. The innovative temperature control system maximizes experimental reproducibility and achieves outstanding accuracy.

  • High cooling and heating rates (3.3°C/s and 4°C/s respectively) for short protocol run times
  • Control accuracy: ±0.1°C
  • Temperature uniformity down to ±0.2°C at 55°C after 15 s
  • Reaction mix reaches set target temperature with best match of set and real data

High-performance smart lid

The heated lid of the QIAamplifier 96 thermal cycler is equipped with smart lid technology with a temperature range from 30°C to 110°C. An integrated slip clutch always maintains constant contact pressure, regardless of shape and height of the plasticware. This optimizes the contact between the sample block well and the walls of the plasticware, resulting in reproducible conditions. A rubber seal in the lid encapsulates the space surrounding the sample block. This closed space increases the sample block temperature uniformity and prevents condensation formation during the final PCR cooling step. A short equilibration period during the first heating of the lid also contributes to a completely balanced atmosphere above the sample block.

Linear Gradient Tool for assay optimization

The Linear Gradient Tool facilitates finding the optimum annealing temperature under experimental conditions. The desired temperature increment for the gradient is programmed after entry of an annealing temperature for the block center. The specific block design and electronics execute a temperature gradient along the long side of the block as linear as technically possible. This means maximum usefulness of the gradient results.

  • Gradient temperature range: 20°C to 99°C
  • Maximum gradient 20°C across the block with 0.1°C increments
  • Control accuracy: ±0.1°C

Robust and designed to last

The sample block of the QIAamplifier 96 is perfectly sealed to prevent condensation from coming into contact with either the Peltier elements located below the sample block or with other electronic components. This grants protection from corrosion and condensation and extends the lifetime of the instrument.

Whisper quiet

The airflow of the QIAamplifier 96 is optimized to keep the maximum noise level of the instrument down to an extremely low 45 decibels. This efficient airflow system also means that the cycler takes up very little space. In addition to the compact footprint, a small clearance zone of just 10 cm is required for sufficient airflow – much lower than that of other thermal cyclers.

Procedure

The QIAamplifier 96 enables fast end-point PCR in tubes, strips and 96-well plate format. The QIAamplifier 96 is designed for convenience, ease of use and reliability and seamlessly integrates any new or existing PCR workflow.

Flexibility of chemistries and plasticware

The QIAamplifier 96 is an open system and can be used in combination with any compatible PCR kit. The SBS sample block allows the QIAamplifier 96 to accommodate most tubes, strips and low-/normal-profile plates. Discover the joy of hassle- and optimization-free PCR with the QIAamplifier 96 and AllTaq Master Mix and PCR Core Kits. Harness the power of multiplexing with UCP Multiplex PCR kits and the QIAamplfier 96. Try the QIAGEN One-Step Ahead RT-PCR Kit with the QIAamplifier 96 for faster one-step RT-PCR with high sensitivity, specificity and fidelity.

Intuitive programming

The QIAamplifier 96 Software is optimized for fast setup of PCR protocols. The Software includes several features for easy programming and intuitive handling:

  • Pre-installed program templates can be directly used for an application or can be adapted easily
  • Multi-step programming allows users to quickly edit all parameters for every program step within a single screen
  • Direct spreadsheet and graphical programming parameters are shown in a single screen; one touch leads from the spreadsheet to the graphical programming mode
Applications

The QIAamplifier 96 is suitable for any end-point PCR application in molecular biology workflows such as genotyping, target detection, semi-quantitative gene expression, reverse transcription and multiplex PCR.

Services

Protect your instrument with diverse service solutions from QIAGEN. Learn more about a specific service agreement to match your needs.

Specifications

Features
Specifications
Connections USB A
Instrument dimensions 260 mm x 430 mm x 210 mm; with open lid 260 mm x 430 mm x 385 mm
Noise level Very low, max. 45 dB
Number of preps per run 96 x 0.2 ml tubes, 1 x 96-well microplate, 12 x 8-well strips
Operating temperature 15°C to 35°C; max. 2,000 m above sea level
Power consumption 550 W
Relative humidity 70 % air humidity
Software User-specific quick start option for 5 recent programs; program preview before start; option for toggling between programming table and graph programming mode; Linear Gradient Tool, service info file (SINF) generation; expanded self-test; adjustable ramp rates; gradient temperature diagram view. Program memory: total capacity of 350 programs in up to 90 user directories
Special components/features High Performance Smart Lid with lid temperature range: 30°C to 110°C
Special feature Automatic restart after power failure
Thermal cycler Block material: Aluminium; Block coating: Special alloy
Thermal performance Ramping: 95°C: ±0.6°C after 15 s; 72°C: ±0.3°C after 15 s; 55°C: ±0.2°C after 15 s; Range: 3°C to 99°C Measured inside the block; Control accuracy: ±0.1°C; Gradient range: 20°C to 99°C; Max./Min. gradient: 20°C/0.1°C; Max. heating rate: 4.0°C/s; Av. heating rate: 3.7°C/s; Max. cooling rate: 3.3°C/s; Av. cooling rate: 3.0°C/s
Touchscreen 7-inch color touchscreen
Voltage 115, 230 Volt, 50/60 Hz
Weight 11.5 kg

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機器ユーザーマニュアル (1)
For molecular biology application. Not for use in diagnostic procedures.
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