Removing highly expressed, but biologically unimportant RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, removal of unwanted RNA species from FFPE samples and from degraded RNA samples is particularly challenging and can result in suboptimal performance.
QIAseq FastSelect is designed for quick, efficient removal of cytoplasmic and mitochondrial rRNA and/or globin mRNA from total RNA during NGS RNA library preparation. QIAseq FastSelect seamlessly integrates with your existing RNA stranded library preparation workflow for RNA removal in a single, 14-minute inline step. Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect removal reagent is directly combined with total RNA and the library preparation-specific buffers. After fragmentation, the reaction temperature is stepwise cooled to room temperature and the remaining library preparation steps are completed. There is no need to perform any type of enrichment on the total RNA samples. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits ensure consistently high performance with RNA amounts ranging from as little as 1 ng to 1 µg. QIAseq FastSelect can be used with RNA from fresh samples, as well as RNA from FFPE (formalin-fixed paraffin-embedded) samples or degraded RNA, and delivers reliable rRNA removal and high reproducibility in downstream applications.