Removing highly expressed, but biologically unimportant RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, removal of unwanted RNA species from full length and fragmented RNA samples can be particularly challenging and can result in suboptimal performance.
QIAseq FastSelect –rRNA Fly Kits are designed for quick, efficient removal of cytoplasmic and mitochondrial fly ribosomal RNA from total RNA during NGS RNA library preparation. QIAseq FastSelect seamlessly integrates with your existing RNA stranded library preparation workflow for RNA removal in a single, 14-minute inline step. Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect removal reagent is directly combined with total RNA and the library preparation-specific buffers. After fragmentation, the reaction temperature is stepwise cooled to room temperature and the remaining library preparation steps are completed. There is no need to perform any type of enrichment on the total RNA samples. QIAseq FastSelect –rRNA Fly Kits ensure consistently high performance with RNA amounts ranging from as little as 1 ng up to 1 μg. QIAseq FastSelect can be used with RNA from fresh samples from many species of fly (dependent upon target rRNA sequence homology with D. melanogaster), and delivers reliable rRNA removal and high reproducibility in downstream applications.