RNeasy PowerSoil Total RNA Kit

For the isolation of high quality total RNA from all soil types

Features

  • High RNA yield from biomass samples by purifying RNA from up to 2 grams of soil
  • High-quality RNA isolation from all soil samples types including difficult environmental samples
  • Effortless removal of PCR inhibitors for highly pure RNA ready for downstream applications
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RNeasy PowerSoil Total RNA Kit

Cat. No. / ID: 12866-25

For the isolation of high quality total RNA from all soil types
$374.00
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The RNeasy PowerSoil Total RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Isolate total RNA from up to 2 grams of soil with the RNeasy PowerSoil Total RNA Kit. Inhibitor Removal Technology removes humic substances and other inhibiting compounds, leaving RNA that is ready for RT-PCR, qPCR and other downstream applications. While the RNeasy PowerSoil Total RNA Kit works with all soil types, it excels with samples that have a high humic substance content such as manure, sediment or compost.

The user-friendly procedures allow all researchers to use the kit. Samples are processed with bead beating for rapid and thorough homogenization. Total RNA is captured in a spin column, washed and eluted, resulting in RNA that is ready to use without further handling or purification. Want to try this solution for the first time? Request a quote for a trial kit.

Note: This kit requires additional phenol, chloroform, and isoamyl alcohol which are not provided.

For co-isolation of DNA from the same starting sample, try the RNeasy PowerSoil DNA Elution Kit.

The RNeasy PowerSoil Total RNA Kit was formerly sold by MO BIO as RNA PowerSoil Total RNA Kit (25).

Specifications

FeaturesSpecifications
Time per run or per prep2.5 hours
Sample size2 g
Throughput1-4 samples
Sample typesAll soil samples, including compost, sediment and manure
ProcessingBead beating
FormatAnion exchange columns
Storage temperatureStore at room temperature (15-30°C)

Resources

Safety Data Sheets (1)
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Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699