QIAprep 96 Plus Kits

For purification of up to 50 μg high-quality plasmid DNA in 96-well format

Features

  • Consistently high yields of up to 50 μg
  • Pure preps suitable for a wide range of applications
  • Vacuum, centrifugation, and automated processing options
undefined

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

QIAprep 96 Plus Miniprep Kit (4)

Cat. No. / ID: 27291

For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.
$1,377.00
ForFor instrument
QIAvac 96
BioRobot Universal
Add to cart
QIAprep 96 Plus Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

QIAprep 96 Plus Kits enable plasmid preparation in 96-well format. Samples can be processed using QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Up to 50 μg of high-quality plasmid DNA (endotoxin levels <10 EU/μg DNA) can be obtained from up to 5 ml of culture.
For optimal results it is recommended to use this product together with QIAvac 96.

Performance

High-purity preps and outstanding yields

QIAprep 96 Plus Kits provide a novel method for preparation of highly pure DNA (see figure " Superior plasmid DNA quality"). Yields of up to 50 μg can be consistently achieved using an optimized procedure. The unique binding buffer provided with the kits ensures very specific binding conditions (see figures "High yields of plasmid DNA" and " Superior performance compared to other suppliers").

See figures

Principle

The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps (see figure " Superior plasmid DNA quality"). Due to unique kit chemistry, up to 50 μg plasmid DNA per well can be obtained from a 5 ml E. coli culture (see figures " High yields of plasmid DNA" and " Superior performance compared to other suppliers").

See figures

Procedure

QIAprep 96 Plus Kits allow plasmid preparation in 96-well format. The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications (see flowchart " QIAprep 96 Plus Miniprep procedure").

Convenient and flexible processing

The QIAprep 96 Plus Miniprep Kit can be conveniently processed using the QIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal System using the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforward plasmid DNA preparation in 96-well format.

See figures

Applications

Plasmid DNA purified using QIAprep 96 Plus Kits is highly suitable for a wide range of applications, including:

  • Preparation of short hairpin vectors (sh-vectors)
  • Enzymatic modifications
  • Cloning
  • Restriction digestion
  • Sequencing

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPreparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing, etc
TechnologyNonchaotropic binding chemistry
Time per run or prep per run40 minutes
Yield15–50 µg
Culture volume/starting material1.25–5 ml culture volume
Samples per run (throughput)48–96 samples per run
Plasmid typeHigh-copy, low-copy, cosmid DNA
ProcessingQIAvac 96, centrifuge, automated

Resources

Kit Handbooks (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.

FAQ

For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?

For the manual procedure, the plates can be partially used. Any unused wells should be sealed with tape. For the BioRobot Universal procedure, the entire 96 sample plate must be used. However, different sized plates may be available from our applications lab.  Please contact QIAGEN Technical Service for more details. 

https://www.qiagen.com/contact/contacttechnicalservice.aspx?WT.svl=m

FAQ ID -2319
Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
For sensitive cell lines and downstream assays, we recommend QIAGEN Plasmid Plus 96 Miniprep Kit, because the endotoxin value is below 1 EU/µg DNA.  The QIAGEN Plasmid Plus 96 MiniPrep kit contains a special protocol for endotoxin removal.  Combination of other chemistries (such as Buffer ER) is not possible with this kit.  For robust cell lines, the QIAprep 96 Plus Miniprep Kit may be okay, as the endotoxin values are below 10 EU/µg DNA.
FAQ ID -2320
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
The QIAprep 96 Plus Miniprep Kit and the QIAGEN Plasmid Plus 96 Miniprep Kit can be used for the purification of plasmids up to 40 kb.
FAQ ID -2316
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
The QIAprep 96 Plus Miniprep Kit and the QIAGEN Plasmid Plus 96 Miniprep Kit can be used for the purification of low-copy plasmids.
FAQ ID -2317
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
Yes, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit are suited for the plasmid purification of short hairpin vectors?
FAQ ID -2318