MagAttract PowerClean DNA Kit

For the automated removal of PCR inhibitors from previously purified DNA using magnetic bead technology

Features

  • Clean, inhibitor-free DNA with ClearMag magnetic bead technology
  • Fast removal of PCR inhibitors from previously purified DNA in under an hour
  • High DNA yield recovery using an automated high-throughput format
MagAttract PowerClean DNA Kit (384)

Cat. No. / ID: 27900-4-KF

For the automated removal of PCR inhibitors from previously purified DNA using magnetic bead technology
$1,440.00
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The MagAttract PowerClean DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Looking for a high-throughput, automated method for cleaning contamination from previously isolated genomic DNA? The MagAttract PowerClean DNA Kit utilizes a combination of ClearMag magnetic bead technology and Inhibitor Removal Technology in an automated, high-throughput format. The combined technologies remove humic substances, polyphenolics, polysaccharides, heme, dyes and other substances from previously isolated samples, providing clean DNA for qPCR and other downstream applications.

The MagAttract PowerClean DNA Kit can process up to 20 μg of previously isolated genomic DNA and uses the same DNA purification chemistry as the DNeasy PowerClean Pro CleanUp Kit to provide a streamlined, hands-free workflow. Cleaned samples from a range of problematic sources including biofilms, stool, plants, water and soil have been validated as providing high-quality DNA. Samples discolored by dyes and high humic acid content can be cleared, allowing accurate DNA quantification by UV-VIS absorption. Cleaned DNA is ready for downstream applications including qPCR and next-generation sequencing.

While the MagAttract PowerClean DNA kit is optimized for use with the Thermo Scientific KingFisher platform, other platforms may be used.

The MagAttract PowerClean DNA Kit was previously sold by MO BIO as the PowerMag DNA Cleanup Kit.

Performance


Resources

Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699