DNeasy PowerLyzer PowerSoil Kit

For the bead-based isolation of DNA from tough soil microbes

Features

  • Optimized to lyse tough microbes and isolate DNA using the PowerLyzer 24 Homogenizer
  • Highly purified DNA produced with Inhibitor Removal Technology that removes humic acid
  • Ready-to-use, high-quality DNA for downstream applications
  • Short and simple protocol for DNA isolation from up to 250 mg samples in just 30 minutes
DNeasy PowerLyzer PowerSoil Kit (50)

Cat. No. / ID: 12855-50

For the bead-based isolation of DNA from tough soil microbes
$322.00
Preparations
50
100
Add to cart
The DNeasy PowerLyzer PowerSoil Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Using novel Inhibitor Removal Technology, extract microbial DNA from all soil types with the DNeasy PowerLyzer PowerSoil Kit. Successful qPCR amplification is possible with isolated DNA of the highest level of purity. Processing a wide variety of environmental samples, especially those with a high humic acid content can be done effectively and efficiently with this kit.


The bead tubes contained in this kit are optimized for bead based homogenizers, particularly the PowerLyzer 24 Homogenizer, also available through QIAGEN. Pure microbial genomic DNA can be easily purified from compost, sediment, manure and other environmental samples and successfully amplified by qPCR. A variety of organisms have been detected from samples purified by the DNeasy PowerLyzer PowerSoil Kit, including both Gram (+/-) bacteria (e.g., Bacillus subtilis, Bacillus anthracis), fungi (e.g., yeasts, molds), algae and actinomycetes (e.g., Streptomyces) and nematodes. Genetic material processed is ready for a variety of downstream applications including next-generation sequencing. 

Isolation of DNA using the DNeasy PowerLyzer PowerSoil Kit can be automated on the QIAcube Connect.

DNeasy PowerLyzer PowerSoil Kit was formerly sold by MO BIO as PowerLyzer PowerSoil DNA Kit.


Specifications

FeaturesSpecifications
sampletypesProcessed soil, fecal, water, food, insects, swabs with PCR inhibitors
processingBead beating
formatSilica Spin Filter Tubes
throughput1-24 samples
timeperrunorperprep30 minutes
bindingcapacityUp to 20 µg per prep
storagetemperatureStore at room temperature(15-30°C)
beadsize0.1 mm glass
samplesize0.25 g

Resources

Kit Handbooks (1)
Webinars (2)
This webinar will focus on the acquisition and development of the preterm gut microbiome from birth and following discharge from intensive care.
This webinar will focus on the automation of QIAGEN’s new line of DNA and RNA sample prep kits for the microbiome.
Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699