AllPrep PowerViral DNA/RNA Kit

For isolating viral or bacterial total nucleic acids from waste water and stool samples

Features

  • Optimized to isolate viral or bacterial DNA and RNA from waste water, stool, biosolids and gut material
  • Inhibitor Removal Technology efficiently eliminates inhibitory substances
  • Highly effective lysis of solid and liquid samples with bead beating protocol 
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AllPrep PowerViral DNA/RNA Kit (50)

Cat. No. / ID: 28000-50

For isolating viral or bacterial total nucleic acids from waste water and stool samples
$448.00
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The AllPrep PowerViral DNA/RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders ✓ Knowledgeable and professional Product & Technical Support ✓ Fast and reliable (re)-ordering

Product Details

The AllPrep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids even from samples high in PCR inhibitors; including waste water, stool, biosolids and gut material. Inhibitor Removal Technology ensures complete removal of inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells that are abundant in stool. The result is DNA and RNA from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-free water and ready to use in PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or next-generation sequencing. Want to try this solution for the first time? Request a quote for a trial kit.

Performance

For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.

Tools to complete your workflow:

The AllPrep PowerViral DNA/RNA Kit was previously sold by MO BIO as the PowerViral Environmental DNA/RNA Kit.

Specifications

FeaturesSpecifications
FormatSilica Spin Filter Columns
Throughput1-24 samples
Binding capacityUp to 40 µg per spin filter
ProcessingChemical lysis and/or bead beating
Storage temperatureStore at room temperature (15-30°C)
Bead size0.1 mm glass (optional)
Time per run or per prep45 minutes
Sample typesProcessed waste water, stool, biosolids and gut material

Resources

Brochures & Guides (1)
Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699