QIAamp DNA Blood Mini Kit

For purification of up to 12 µg genomic, mitochondrial, or viral DNA from blood and related body fluids

Rapid purification of high-quality, ready-to-use DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors for reliable results

The QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification. The QIAamp DNA Blood Mini Kit is designed for processing up to 200 μl fresh or frozen human whole blood. QIAamp Mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Purification of DNA using the QIAamp DNA Blood Mini Kit can be fully automated on the QIAcube Connect.

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Product Details
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Cat No./ID: 51104

QIAamp DNA Blood Mini Kit (50) Add bundle to the cart

$172.00

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For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)

Buy QIAamp DNA Blood Mini (50) with QuantiNova Multiplex PCR Kit (100)

Bundle with QuantiNova Multiplex PCR Kit (100)

Cat No./ID: 51106

QIAamp DNA Blood Mini Kit (250) Add bundle to the cart

$741.00

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For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)

Buy QIAamp DNA Blood Mini (250) with QuantiNova Multiplex PCR Kit (100)

Bundle with QuantiNova Multiplex PCR Kit (100)

QIAamp DNA Blood Mini Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Paternity testing by RFLP analysis.

The QIAcube.

Long-range PCR.

Apoptotic banding in stored blood.

QIAamp spin column procedure.

Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

Procedure

The QIAamp DNA Blood Mini Kit simplifies isolation of DNA from blood and related body fluids with fast spin-column or vacuum procedures (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl, with a preparation time of 20–40 minutes. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. The QIAamp DNA Blood Mini Kit procedure is automatable (see figure "QIAcube").

QIAamp sample preparation technology is fully licensed.

Vacuum processing

With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Applications

The QIAamp DNA Blood Mini Kit provides proven QIAamp technology for purification of DNA from a variety of materials using Mini spin columns with 2.0 ml collection tubes. Sample sources include:

Fresh and frozen whole blood or buffy coat

Plasma or serum

Bone marrow

Lymphocytes

Platelets

Body fluids

Specifications

Features	Specifications
Applications	PCR, long-range PCR, Southern blotting
Elution volume	50–200 µl
Format	Spin column
Main sample type	Whole blood, body fluids
Processing	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA, mitochondrial DNA, viral DNA
Sample amount	1–200 µl
Technology	Silica technology
Time per run or per prep	20–40 minutes
Yield	4–12 µg

Product Resources

Application Notes (1)

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	(EN) - Stable 16-year storage of DNA purified with the QIAamp DNA Blood Mini Kit	Show details

Brochures & Guides (4)

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	Analyzing Genetic Differences - (EN) Second edition — innovative tools	Show details
	Introducing QIAseq Accelerate your NGS performance through Sample to Insight solutions	Show details
	ZH-QIAGENs-Preanalytic-NGS-Solutions	Show details
	Product Profile - QIAamp® genomic DNA kits	Show details

FAQs (20)

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	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	View
	When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit? FAQ ID -100	View
	Are QIAamp DNA isolation kits suitable for apoptosis studies? FAQ ID -149	View
	What is the cellular composition of human blood? FAQ ID -2951	View
	How can I precipitate genomic DNA using isopropanol? FAQ ID -2953	View
	How do I perform a DNA precipitation to concentrate my sample? FAQ ID -305	View
	How can QIAGEN Protease and Proteinase K be inactivated? FAQ ID -315	View
	What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield? FAQ ID - 3516	View
	Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)? FAQ ID - 3518	View
	Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit? FAQ ID -518	View
	Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits? FAQ ID -563	View
	Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL? FAQ ID -633	View
	What is the average amount of DNA and RNA present in 1 ml normal serum? FAQ ID -635	View
	What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728	View
	What is the composition of buffer AE? FAQ ID -730	View
	Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA? FAQ ID -754	View
	What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761	View
	Do you have a protocol for Streptokinase treatment of clotted blood? FAQ ID -912	View
	Do you have a protocol for detection of HBV DNA by PCR? FAQ ID -915	View
	Do you have a protocol for isolation of genomic DNA from saliva and mouthwash? FAQ ID -917	View

Scientific Posters (1)

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	Rapid and sensitive detection of bacillus anthracis by real-time PCR - (EN)	Show details

Supplementary Protocols (3)

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	Purification of genomic DNA from the PAXgene® Blood ccfDNA Tube using the QIAamp® DNA Blood Mini Kit - (EN)	Show details
	Isolation of genomic DNA from saliva and mouthwash using the QIAamp® DNA Blood Mini Kit; spin procedure - (EN)	Show details
	Isolation of genomic DNA from saliva and mouthwash using the QIAamp® DNA Blood Mini Kit; vacuum procedure - (EN)	Show details

Quick-Start Protocols (1)

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	Quick Start Protocol: QIAamp Blood Mini Kit	Show details

User-Developed Protocols (1)

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	Streptokinase treatment of clotted blood - (EN)	Show details

Kit Handbooks (1)

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	QIAamp DNA Mini Blood Mini Handbook - EN	Show details

Safety Data Sheets (3)

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	MSDS QIAamp DNA Blood Mini Kit (50)
	MSDS QIAamp DNA Blood Mini Kit (250)
	CofA

Product citations in scientific articles

See more details on Bioz

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