The kits use a fast procedure involving pre-concentration of circulating nucleic acids onto magnetic beads and a cleanup of the resulting pre-eluate on QIAamp MinElute columns in a quick and convenient spin procedure. The robust procedure helps to eliminate sample-to-sample cross-contamination and increases user safety when handling potentially infectious samples.
The simple procedure, which is highly suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 24 samples. Free-circulating DNA is eluted in Ultra-clean Water, ready for use in downstream reactions or storage a –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities. The procedure can be partially automated on the QIAcube