EZ1 DSP Virus Kit

For automated, simultaneous purification of viral nucleic acids and bacterial DNA from 1–6 or 1–14 human biological specimens using the EZ1 Advanced or the EZ1 Advanced XL

Features

  • High analytical sensitivity even with low viral titers
  • Rapid and flexible purification of 1–6 or 1–14 samples per run
  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results

Useful Resources

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EZ1 DSP Virus Kit (48)

Cat. No. / ID: 62724

For 48 viral nucleic acid preps: Prefilled Reagent Cartridges, Disposable Tip-Holders, Disposable Filter-Tips, Sample Tubes, Elution Tubes, buffers, carrier RNA
$475.00
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The EZ1 DSP Virus Kit is intended for in vitro diagnostic use.

Product Details

The EZ1 DSP Virus Kit contains all required reagents and labware for automated purification of viral nucleic acids and bacterial DNA from human serum, plasma, cerebrospinal fluid (CSF), urine, whole blood, stool, transport media, respiratory samples, and dried swabs using magnetic-particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the EZ1 Advanced or the EZ1 Advanced XL. The EZ1 DSP Virus Kit provides exact performance specifications, assuring highly reliable viral nucleic acid purification.

Principle

Automated sample preparation enables efficient, standardized purification of nucleic acids. The EZ1 Advanced and the EZ1 Advanced XL enable purification of nucleic acids using proven magnetic-particle technology for use in routine in vitro diagnostic applications. Reagent production is subjected to stringent quality control assuring reliable and robust performance.
An easy-to-use instrument, prefilled and sealed reagent cartridges, and simple protocol selection and worktable setup help to prevent handling errors and allow the system to be operated by anyone — from the novice to the expert. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. Purified viral DNA and RNA are ready to use in sensitive downstream diagnostic assays based on enzymatic amplification, such as PCR and reverse transcriptase PCR (RT-PCR) for viral load monitoring.

Procedure

The EZ1 Advanced or the EZ1 Advanced XL in combination with the proven EZ1 DSP Virus Kit enables purification of viral nucleic acids and bacterial DNA from 1–6 (EZ1 Advanced) or 1–14 (EZ1 Advanced XL) samples of human serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swabs. All processing steps are performed by the instrument, from piercing the reagent cartridge to elution of highly pure nucleic acids. The innovative design of the instrument means that the pipet tips function as separation chambers, increasing the efficiency of magnetic purification and eliminating the need for centrifugation steps. The streamlined procedure provides robust walkaway purification of nucleic acids with minimal hands-on time.

Applications

In combination with the EZ1 Advanced DSP Virus Card and the EZ1 Advanced or the the EZ1 Advanced XL DSP Virus Card and the EZ1 Advanced XL, the EZ1 DSP Virus Kit provides a fully automated procedure for simultaneous purification of viral nucleic acids and bacterial DNA from human serum, plasma, CSF, urine, whole blood, stool, transport media, respiratory samples, and dried swabs. Viral nucleic acids purified using the EZ1 DSP Virus Kit are ready to use in downstream diagnostic assays based on enzymatic amplification, such as PCR and RT-PCR.

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time RT-PCR, genotyping
Elution volume75–50 µl
Main sample typeSerum, plasma
FormatSample tube
CE/FDA/IVD compatibleCE/IVD
ProcessingAutomated
For automated processingBioRobot EZ1
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Sample amount100–400 µl
TechnologyMagnetic particles
Time per run or per prep40 minutes
YieldVaries

Resources

Kit Handbooks (1)