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JAK2 RGQ PCR Kit RUO

For quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR

  • Reliable and sensitive quantification of JAK2 V617F
  • Larsen’s primers in line with most recent international technical recommendations (1)
  • Internal control included
  • All-in-one, ready to use solutions including QIAGEN master mix
  • Easy-to-use protocols validated on the QIAsymphony SP and the Rotor-Gene Q 5plex HRM
  • Optimized turn-around time

The JAK2 RGQ PCR is intended for real-time PCR on the Rotor-Gene Q 5plex HRM. The kit provides all the reagents optimized for reliable and sensitive detection, and quantification of the JAK2 V617F/G1849T mutation in genomic DNA.

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Cat No./ID: 673613
JAK2 RGQ PCR Kit (24) RUO
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For 24 reactions: V617F Positive Control, V617F Negative Control, 2 sets of standards plasmid dilutions JAK2 wild-type and JAK2 V617F, qPCR master-mix, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F including internal control
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

References:

  1. Jovanovic, J.V.et al. (2013) Establishing optimal quantitative-polymerase chain reaction assays for routine diagnosis and tracking of minimal residual disease in JAK2-V617F-associated myeloproliferative neoplasms: a joint European LeukemiaNet/MPN&MPNr-EuroNet (COST action BM0902) study. Leukemia 27, 2032.

Product Details

Performance

To ensure improved sensitivity and reproducibility, the JAK2 RGQ PCR Kit has been optimized to detect the JAK2 V617F/G1849T mutation and uses prediluted plasmid standards, primers, and probe mixes in line with recent international technical recommendations (1). The limit of detection (LOD) is 0.042%.

Principle

The JAK2 RGQ PCR Kit is a ready-to-use kit for the detection and quantification of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA. The kit use polymerase chain reaction (PCR) and amplification refractory mutation system (ARMS) technology on Rotor-Gene Q 5plex HRM.

The kit is highly sensitive with excellent reproducibility. The JAK2 RGQ PCR Kit includes 4 plasmid standard dilutions for each mutant JAK2 V617F and wild-type JAK2 allele. Use of these standards enables the accurate quantification of JAK2 V617F and JAK2 wild-type. Results are expressed as the percentage of JAK2 V617F in total JAK2, as well as mutation detection status based on the LOD.

 

Procedure

The JAK2 RGQ PCR Kit allows detection and quantification of the JAK2 V617F/G1849T mutation on Rotor-Gene Q 5plex HRM. The first step is manual or QIAsymphony-automated DNA extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of the genomic DNA by real-time PCR, and all the reagents needed for this step are provided in the kit. Simply start the reaction on the Rotor-Gene Q 5plex HRM using the optimized protocols as described in the kit handbook.

 

Applications

The JAK2 RGQ PCR Kit enables sensitive and reliable detection and quantification of the JAK2 V617F/G1849T mutation. This kit is for research use only.

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Package Insert (1)
Important Note for JAK2 RGQ PCR Kit, catalog no. 673613
For more information about this product, please refer to JAK2 RGQ PCR Kit Handbook (www.qiagen.com/JAK2).
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