The importance of rRNA removal in RNA-seq optimization
QIAseq FastSelect: Increase your unique reads, discover more biology
Did you know that a critical step for RNA-seq optimization is efficient removal of rRNA? The amount of rRNA removed is directly proportional to the number of unique gene reads obtained from your library. Efficient, near complete rRNA removal prior to sample cDNA synthesis ensures you aren't making unwanted ribosomal cDNA. Introduce our game-changing QIAseq FastSelect technology into your workflow after performing your RNA-seq library preparation protocol and experience highly efficient rRNA removal in as little as 14 minutes. In fact, samples treated with QIAseq FastSelect don't require pre-sequencing PCR amplification – simply use your library as is, save even more time and obtain a higher number of unique gene reads. FastSelect your sample before using your library preparation kit and discover more biology.
QIAseq FastSelect and SARS-CoV-2
Unravel host-microbiome interactions to advance coronavirus metatranscriptomics research
Working with mixed human and microbiome samples from nasopharyngeal, lung and other tissues for coronavirus metatranscripomics studies? To maximize unique mRNA/gene expression reads, use QIAseq FastSelect –rRNA HMR Kits and QIAseq FastSelect –5S/16S/23S Kits separately or in combination for fast, effective and targeted removal of both host and bacterial rRNA – while still retaining viral RNAs.
Recent SARS-CoV-2 publications using QIAseq FastSelect:
New! QIAseq FastSelect Epidemiology KitThe QIAseq FastSelect Epidemiology Kit simplifies fast, efficient rRNA removal from mixed human, mouse or rat and bacterial samples, prior to RNA-seq library prep. This cost-effective kit enables high-throughput processing for mixed sample rRNA removal in less than one hour.
New! QIAseq FastSelect Kits for developmental biology model organismsThe QIAseq FastSelect product line is now extended to include dedicated kits for Drosophila (fly) and C. elegans (worm) Danio rerio (zebrafish) and Salmo solar (salmon) developmental biology model organisms. Remove highly abundant rRNA of low scientific value from fly, worm or fish RNA-seq libraries in just 14 minutes using a single step. Depending on rRNA sequence homology, QIAseq FastSelect −rRNA Fly, QIAseq FastSelect –rRNA Worm and QIAseq FastSelect −rRNA Fish Kits may remove rRNA from samples obtained from additional related species.
QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin KitsPerforming RNA-seq with mammalian tissue, organ or liquid biopsy samples? Our QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits are single kit solutions that allow you to achieve >95% of rRNA and globin mRNA removal from human, mouse, rat and other mammalian samples. With a 30% faster protocol than our previous kit versions, QIAseq FastSelect enables RNA removal using just a single 10-second pipetting step, with only 14 minutes of incubation. Combine with the QIAseq Stranded Total RNA Lib Kit, a high-quality, stranded RNA library prep kit with maximum transcript coverage, enabling detection of even low-expression RNA molecules.
QIAseq FastSelect –rRNA Plant KitsIf you’re working with agricultural and environmental RNA-seq samples, removal of unwanted plant rRNA can increase the sensitivity of your RNA-seq, allowing you to maximize usable reads and cost-effectively generate transcriptome-wide data. New QIAseq FastSelect –rRNA Plant Kits enable removal of cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and chloroplast (4.5S, 5S, 16S and 23S) rRNA from your plant RNA samples in just 14 minutes. Combine with the QIAseq Stranded Total RNA Lib Kit for robust, strand-specific RNA-seq library prep with maximum coverage.
QIAseq FastSelect –rRNA Yeast KitsMaximize the usable RNA-seq reads you generate in applications such as fermentation studies and gene expression analysis by effectively removing unwanted yeast rRNA. New QIAseq FastSelect –rRNA Yeast Kits enable efficient removal of yeast cytoplasmic (35S [made up of 25S, 18S and 5.8S], 25S, 18S, 5.8S and 5S) and mitochondrial (21S, 15S, ACI60_gr01 [large subunit ribosomal RNA] and ACI60_gr02 [small subunit ribosomal RNA]) rRNA in just 14 minutes. For subsequent stranded library prep, combine with the QIAseq Stranded Total RNA Lib Kit for sensitive detection of low-expression RNA molecules.
QIAseq FastSelect –5S/16S/23S KitsHighly abundant bacterial 5S,16S and 23S rRNA wastes precious sequencing resources and reduces the number of unique reads you obtain from your RNA-seq library. New QIAseq FastSelect –5S/16S/23S Kits are a revolutionary solution for pan-bacterial 5S,16S and 23S rRNA removal from fragmented or full-length RNA for metatranscriptomics studies. No need for capture or enzymatic digestion. Simply use our 14-minute protocol, followed by bead cleanup for removal of unwanted rRNA from complex bacterial samples. Then move on to stranded, high-quality library prep using the QIAseq Stranded Total RNA Lib Kit for sensitive detection of low-expression RNA molecules with increased complexity and transcript coverage.
QIAseq FastSelect success stories
Evolution of new technologies for mammalian, bacterial, plant and yeast samples
Speaker: Jonathan Shaffer, Ph.D., M.B.A., Director, R&D, NGS assays, QIAGEN
On-demand recorded webinar
rRNA removal for RNA-seq applications involving viruses, host responses and metatranscriptomics
Speaker: Samuel Rulli, Ph.D., Associate Director, RNA-seq profiling, NGS assay technologies, QIAGEN
On-demand recorded webinar