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Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?
FAQ ID -2783
View
On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
View
What are the advantages of using the SureSilencing shRNA Plasmids over siRNA?
FAQ ID -2786
View
Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays?
FAQ ID -2747
View
The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750
View
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
FAQ ID -2766
View
The pathway reporter luciferase activity values are less than the negative control, is there a problem?
FAQ ID -2767
View
How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays?
FAQ ID -2742
View
How can RNA interference be used as a life science research tool?
FAQ ID -2756
View
How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system?
FAQ ID -2731
View
How can I normalize my EpiTect ChIP results?
FAQ ID -2751
View
What is the recommended amount of input template for each RT² qPCR Primer Assay?
FAQ ID -2716
View
What is the RT² Profiler PCR Array?
FAQ ID -2718
View
Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples?
FAQ ID -2732
View
Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays?
FAQ ID -2765
View
Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region?
FAQ ID -2744
View
Can I use your EpiTect One-Day kit with transcription factors?
FAQ ID -2752
View
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
View
Can I manually set the threshold line?
FAQ ID -2702
View
Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples?
FAQ ID -2733
View
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
View
Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays?
FAQ ID -2745
View
Can I use your EpiTect ChIP system in a RNA study?
FAQ ID -2748
View
Can I use total RNA for the miRNA PCR Arrays or Assays?
FAQ ID -2726
View
Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology?
FAQ ID -2738
View
Can I transform the Cignal Reporter Assays?
FAQ ID -2762
View
Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene?
FAQ ID -2782
View
How many experiments can I do with the Cignal Lenti Reporter Assays?
FAQ ID -2769
View
How many housekeeping genes are included in each PCR Array?
FAQ ID -2704
View
How reliable are the results from the miScript Primer Assays?
FAQ ID -2730
View
What are the advantages of EpiTect ChIP qPCR Assays and Arrays?
FAQ ID -2721
View
What are EpiTect ChIP qPCR Assays?
FAQ ID -2720
View
How do I choose EpiTect ChIP-grade antibodies?
FAQ ID -2746
View
How do you determine the efficiency using the PCR array?
FAQ ID -2701
View
How does QIAGEN control the quality of the RT² qPCR Primer Assays?
FAQ ID -2711
View
How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays?
FAQ ID -2761
View
Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
View
What are the storage conditions for the Microbial DNA qPCR products?
FAQ ID — 3394
View
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
View
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
View
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
View
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
View
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
View
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
View
What sample types can be tested on the arrays/assays?
FAQ ID — 3399
View
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
View
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
View
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
View
View
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
View
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
View
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
View
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
View
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
View
3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
View
3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
View
3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
View
3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
View
View
3325 - What are the catalog numbers for the packing materials for the QIAcube?
View
3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
View
3336 - Can I use the linearized DNA for Epitect Bisulfite treatment with the Epitect Bisulfite kit?
View
3337 - Which method is used for QIAGEN sequencing service?
View
3338 - Does the miRNeasy Serum/Plasma Spike-in Control (cat n° 219610) have 3'-hydroxyl and 5'-phosphate groups?
View
3342 - How is the unmethylated unconverted EpiTect DNA control to be used?
FAQ - 3342
View
3343 - Why is it difficult to quantify DNA using UV/Vis spectroscopy and fluorescence methods following EpiTect Bisulfite Conversion? How best do you quantify it?
FAQ - 3343
View
3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
View
3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
View
3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
View
3320 - What is the sensitivity of the Type-it HRM PCR Kit?
View
3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
View
3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
View
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3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
View
3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
View
3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
View
3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
View
3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
View
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID -499
View
3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
View
3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
View
3302 - How long can I store my purified RNA?
View
Can I use amplified genomic DNA with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3415
View
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
FAQ ID - 3479
View
Do you have stability data for RNA isolated with PAXgene miRNA Kit?
FAQ ID - 3480
View
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
FAQ ID - 3481
View
Can I use genomic DNA from fixed samples with qBiomarker Copy Number PCR Arrays?
FAQ ID — 3416
View
Can the PAXgene Blood DNA Tubes be stored at 4°C?
FAQ ID - 3493
View
How should I thaw frozen PAXgene Blood DNA Tubes?
FAQ ID - 3495
View
Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
FAQ ID - 3491
View
3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?
FAQ ID - 3497
View
Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
FAQ ID - 3498
View
Does the DNA extracted using the PAXgene Blood DNA System contain RNA?
FAQ ID - 3499
View
Can I multiplex the Microbial DNA qPCR Assays?
FAQ ID — 3408
View
Can I measure virulence factor gene expression?
FAQ ID — 3405
View
What sample types can I test on qBiomarker Copy Number PCR Arrays?
FAQ ID — 3423
View
Which probe labels are available for the Microbial DNA qPCR Assays?
FAQ ID — 3407
View
What is the sensitivity for the Microbial DNA qPCR kits?
FAQ ID — 3409
View
Are the Microbial DNA qPCR Assays wet-lab verified?
FAQ ID — 3406
View
What sequences are used to design the Microbial DNA qPCR Assays?
FAQ ID — 3403
View
Can I measure antibiotic resistance gene expression?
FAQ ID — 3404
View
Are the assays species-specific?
FAQ ID — 3400
View
Why is the copy number call lower than expected?
FAQ ID — 3413
View
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
View
How should I analyze the data generated from a qBiomarker Copy Number PCR Array experiment?
FAQ ID — 3421
View
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
View
What qPCR mastermix should I use with the qBiomarker Copy Number PCR Arrays and Assays
FAQ ID — 3422
View
What could have gone wrong if the CT values are unusually high for all wells in a sample?
FAQ ID — 3412
View
How much DNA should I use on a qBiomarker Copy Number PCR Array?
FAQ ID — 3417
View
How much DNA should I use in a qBiomarker Copy Number PCR Assay?
FAQ ID — 3418
View
What testing should be performed in order to assess the quality of a DNA sample?
FAQ ID — 3424
View
How do you choose which assays to include on the arrays?
FAQ ID — 3420
View
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
View
How many samples can be processed with the PAXgene Blood miRNA Kit?
FAQ ID - 3477
View
As part of my RT procedure I routinely heat an aliquot of the eluate prior to the RT reaction. Do I still need to heat the whole eluate after the RNA preparation?
FAQ ID - 3468
View
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
View
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?
FAQ ID - 3460
View
I need to buy a new computer for my PyroMark Q96. Can I reinstall the PyroMark Q96 software on my new computer or do I need to purchase a new license?
FAQ ID - 3472
View
Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
FAQ ID - 3494
View
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?
FAQ ID - 3474
View
Can I reinstall the PyroMark Q24 software on my new computer or following an operating system upgrade, or do I need to purchase a new license?
FAQ ID - 3473
View
What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID - 3475
View
Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID - 3476
View
2. Are there any special considerations for the collection of blood using PAXgene Blood DNA Tubes CE (e.g., use of a blood collection set or discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3485
View
Are the PAXgene Blood DNA Tubes sterile?
FAQ ID - 3487
View
What is the additive in PAXgene Blood DNA Tubes
FAQ ID - 3486
View
What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
FAQ ID - 3482
View
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
FAQ ID - 3483
View
How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
FAQ ID - 3489
View
Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
FAQ ID - 3484
View
How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
FAQ ID - 3492
View
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
View
What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
FAQ ID - 3488
View
How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
FAQ ID - 3490
View
Can the miScript II RT Kit be used to quantify piRNAs?
FAQ ID - 3432
View
Can the miScript Plant RT Kit be used to quantify animal miRNAs?
FAQ ID - 3433
View
Can the miScript Plant RT Kit be used to quantify piRNA expression?
FAQ ID - 3434
View
Does the miScript Plant RT Kit contain controls?
FAQ ID - 3435
View
Are any of the miScript II RT Kit components interchangeable with the miScript Plant RT Kit components?
FAQ ID - 3436
View
What is the composition of the 5x miScript Plant RT Buffer?
FAQ ID - 3437
View
What should I use to isolate total RNA from plant tissues and what is the maximum recommended amount of tissue input?
FAQ ID - 3438
View
Are miScript Primer Assays available for plant mature miRNAs?
FAQ ID - 3439
View
Are plant miScript Primer Assay designs wet-bench validated?
FAQ ID - 3440
View
Are miScript Primer Assay included in miScript miRNA PCR Arrays the same primers as the miScript Primer Assays available in individual tubes?
FAQ ID - 3441
View
Can you describe the adaptor found in the 10x miScript Plant Adaptor?
FAQ ID - 3442
View
What is the amplicon size of PCR products generated with the miScript Plant qPCR System?
FAQ ID - 3443
View
Can cDNA synthesized with the miScript Plant RT Kit be used in real-time PCR with TaqMan® MicroRNA Assays?
FAQ ID - 3445
View
What is the recommended RNA and RNA input range for the miScript Plant RT Kit?
FAQ ID - 3446
View
Can I scale up reagents if I want to use more sample with the MagAttract HMW DNA Kit (cat. no. 67563)?
FAQ ID - 3448
View
Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach?
FAQ ID - 3469
View
How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays?
FAQ ID — 3411
View
What is the difference between the Microbial DNA qPCR Assays and the QuantiFast Pathogen +IC Kits?
FAQ ID — 3410
View
Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
FAQ ID - 3471
View
What RNA yields are expected with the PAXgene Blood RNA kit?
FAQ ID - 3464
View
What are possible reasons for blood draws with lower than expected blood volume?
FAQ ID - 3462
View
How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C?
FAQ ID - 3463
View
Can the PAXgene Blood RNA System be used to isolate DNA?
FAQ ID - 3466
View
Can the PAXgene Blood RNA System be used to isolate viral RNA?
FAQ ID - 3465
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