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Read more about GeneReader NGS System
The first complete Sample to Insight NGS solution.

RNeasy PowerWater Kit

For the isolation of total RNA from filtered water samples

Features

  • Rapid RNA isolation from water samples in less than 40 minutes
  • Highly pure RNA extraction in turbid water samples from all water-borne microorganisms
  • Ready-to-use, inhibitor-free RNA for downstream applications
RNeasy PowerWater Kit (50)

Cat. No. / ID: 14700-50-NF

For the isolation of total RNA from filtered water samples, including turbid water
The RNeasy PowerWater Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Isolate RNA from all microbes present in environmental water samples with the RNeasy PowerWater Kit. The kit is designed to isolate RNA from bacteria (Gram +/-), algae and fungi. Samples are processed from start to finish, including the RNase step, in 40 minutes. The kit combines mechanical lysis and Inhibitor Removal Technology to yield high-quality, ready-to-use RNA, even with samples containing high amounts of salts, metal, humic acids and other organic materials. Resulting RNA can then be used in RT-PCR, qPCR, RNA-seq and other downstream applications. Want to try this solution for the first time? Request a quote for a trial kit.

RNeasy PowerWater Kit was formerly sold by MO BIO as PowerWater RNA Isolation Kit.

Specifications

FeaturesSpecifications
formatSilica spin filter tubes
throughput1-6 samples
timeperrunorperprep40 minutes
processingBead beating
storagetemperatureStore lyophilized DNase I at 4°C All other kit reagents and components should be stored at room temperature (15-30°C)
sampletypesOcean water, fresh water, brackish water, ground water, waste water
beadsize0.15 mm, 0.7 mm garnet mix
bindingcapacityUp to 40 µg per prep

Resources

Quick-Start Protocols (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699