QIAsymphony DNA Kits

For automated purification of DNA from 1–96 samples

Features

  • Purification of DNA from a broad range of sample types
  • Novel, prefilled reagent cartridges for ease of use
  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents

Products

QIAsymphony DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
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QIAsymphony DNA Midi Kit (96)

Cat. No. / ID: 931255

For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
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QIAsymphony DNA Mini Kit (192)

Cat. No. / ID: 931236

For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories

Product Details

QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Performance

Automated purification of DNA on the QIAsymphony SP provides high yields of DNA (see figure " High yields of DNA from human whole blood"). Spectrophotometric analysis showed that the DNA was highly pure (see figure " Highly pure DNA"). DNA gave high-performance results in downstream PCR of a single-copy gene with no inhibition of PCR observed with 1 µl or 10 µl eluate in the amplification reaction (see figures " High-performance results in PCR — A" and " High-performance results in PCR — B").
See figures

Principle

QIAsymphony DNA Kits combine the speed and efficiency of silica-based purification of genomic DNA with the convenient handling of magnetic particles (see figure " Efficient processing of magnetic particles"). Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.
See figures

Procedure

For tissues, cultured cells, and bacteria protocols, manual sample pretreatment is required. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.

QIAsymphony DNA protocols
Protocol         Kit  Sample material Processed  volume
DNA Blood 200       QIAsymphony DNA Mini Kit (192) Human whole blood 200 μl
DNA Blood 400      QIAsymphony DNA Midi Kit (96)  Human whole blood 400 μl
DNA Blood 1000     QIAsymphony DNA Midi Kit (96) Human whole blood 1000 μl
DNA Buffy Coat 200   QIAsymphony DNA Mini Kit (192) Buffy coat 200 μl 
DNA Buffy Coat 400     QIAsymphony DNA Midi Kit (96)  Buffy coat   400 μl 
Tissue LC 200  QIAsymphony DNA Mini Kit (192)  Pretreated sample lysates from tissue, FFPE tissue, cultured cells, and bacterial cultures; for example: heart (25 mg), lung (25 mg), kidney (25 mg), muscle (50 mg), Jurkat cells (2 x 106cells)* 200 μl
Tissue HC 200  QIAsymphony DNA Mini Kit (192)  Pretreated sample lysates from tissue, cultured cells, and bacterial cultures; for example: spleen (25 mg), liver (25 mg), Jurkat cells (1 x107 cells), bacteria (4 x 109 cells)* 200 μl
Virus Blood 200  QIAsymphony DNA Mini Kit (192) Human whole blood  200 μl
* Values in brackets indicate the recommended maximum sample size.

Applications

DNA purified using QIAsymphony DNA Kits is of high quality and is ready for use in downstream applications, such as:

  • PCR and real-time PCR
  • Genotyping, STR, and SNP analysis
  • Sequencing

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsPCR, Real-Time PCR, Genotyping
sampletypeBlood; Cell culture (only Mini Kit); Tissue (only Mini Kit)
elutionvolumeMini Kit: 50-200 µl; Midi Kit: 100-500 µl
sampleamountMini Kit: 200 µl blood, up to 50 mg tissue or up to 1 x 10e7 cells; Midi Kit: 400 µl or 1000 µl blood
yieldMini Kit: 4-10 µg from 200 µl blood; Midi Kit: 8-20 µg from 400 µl blood or 15-50 µg from 1000 µl blood

Resources

Application/Protocol Documents (6)
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
For use with software version 4.0 or higher 
Labware Files and Documents (1)
For use with software version 5.0 or higher 

FAQ

Can I program my own protocols for the QIAsymphony SP?

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

 

 

 

FAQ ID -1904
Can uneven sample numbers be processed on the QIAsymphony SP module?

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

 

 

FAQ ID -1906
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?

No. We do not recommend use of carrier RNA with the QIAsymphony Virus Blood 200 Protocol.

 

FAQ ID -2163
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?

In general, magnetic particles are not carried over into QIAsymphony eluates. However, if high amounts of genomic DNA are purified (large number of white blood cells in the sample) magnetic-particle carryover may be observed. If carryover does occur, magnetic particles in eluates will not affect most downstream applications. If magnetic particles need to be removed before performing downstream applications, tubes or plates containing eluates should first be placed in a suitable magnet and the eluates transferred to a clean tube.

 

FAQ ID -2166
How should DNA isolated on the QIAsymphony be stored?

Eluates obtained on the QIAsymphony SP can be stored at 2–8°C for 3 days before use in downstream applications and should be placed at –20°C or –80°C for long-term storage.

 

 

FAQ ID -2165
What are the expected DNA yields from tissue processed on the QIAsymphony SP?

DNA yields strongly depend on the type and source of the tissue processed on the QIAsymphony SP. Approximately 70–80 µg DNA can be isolated from 25 mg of spleen, 40–50 µg from 25 mg liver, and 10–15 µg from 50 mg muscle.

 

FAQ ID -1919
What sample input volumes can be used on the QIAsymphony SP?
200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.
FAQ ID -1907
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?

The maximum amount of tissue that can be processed on the QIAsymphony SP using QIAsymphony DNA Mini Kit is 50 mg, and the maximum number of cultured cells is 1 x 107.

See details for upper limits of various starting materials in the table below:

 

Sample type Sample amount Protocol
Heart 25 mg Tissue Standard
Spleen 25 mg Tissue High Content
Lung 25 mg Tissue Standard
Liver 25 mg Tissue High Content
Kidney 25 mg Tissue Standard
Muscle 50 mg Tissue Standard
Mouse tail 0.8 cm Tissue Standard
Jurkat Cells 2 x 10e6 Tissue Standard
Jurkat Cells 1 x 10e7 Tissue High Content

FAQ ID -1917
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?

A total of 192 samples can be processed with one QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol (96 samples per reagent cartridge).

 

 

 

FAQ ID -2159
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?

Sample volumes of 200 µl can be processed with the Virus Blood 200 Protocol using the QIAsymphony DNA Mini Kit.

 

 

FAQ ID -2156
What sample volumes can be used with the tissue HC and LC protocols on the QIAsymphony SP?
After pretreatment, 220 µl of the lysates must be transferred to the QIAsymphony SP to ensure that 200 µl of sample is processed.
FAQ ID -2992
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.
FAQ ID -2993
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?

Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using QIAsymphony DNA Kits.

Samples are lysed by Proteinase K digestion in lysis buffers optimized for tissue, cultured cells, and bacteria, respectively. For lysis of gram-positive bacteria, lysozyme is used in addition to Proteinase K. Lysed samples are homogenized by pipetting up and down, and insoluble material is removed by centrifugation (see the QIAsymphony DNA Handbook for details).

 

FAQ ID -1916
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?

Samples should be processed on the QIAsymphony within 24 hours of collection. Samples should be transported and stored at 2–25°C.

 

 

 

FAQ ID -2157
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?

Human whole blood samples treated with EDTA or citrate can be processed using the QIAsymphony Virus Blood 200 protocol with the QIAsymphony DNA Mini Kit. We do not recommend using heparin-treated blood samples.

 

 

 

FAQ ID -2154
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?

Yes. The DNA Tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.

 

 

 

 

FAQ ID -1921
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?

Yes. QIAsymphony DNA Investigator Kit protocols purify both genomic and mitochondrial DNA.

 

 

 

FAQ ID -2030
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?

Final elution volumes of 60 µl, 85 µl, 110 µl, or 165 µl can be selected using the QIAsymphony Virus Blood 200 protocol. Please note that the initial elution volumes are 95 µl, 120 µl, 145 µl, or 200 µl. When calculating the amount of internal control(s) as well as the titer of the processed sample, it is necessary to take into consideration the initial volume of elution buffer that is used for each sample.

 

FAQ ID -2160
Is it possible to modify QIAsymphony protocols?

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

 

FAQ ID -1903
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?

The High Content protocol for DNA extractions on the QIAsymphony SP is optimized for samples with an expected DNA yield of more than 30 µg. Up to 90 µg DNA can be purified from tissues with high cell densities (e.g., spleen) or large numbers of cultured cells.

 

FAQ ID -1918
What is the range of elution volumes on the QIAsymphony SP?

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

 

 

 

FAQ ID -1908
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?

Various types of tissue, cultured cells, and bacterial pellets can be processed using the tissue protocols for the QIAsymphony DNA Kits on the QIAsymphony SP.

 

 

 

 

FAQ ID -1915
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?

No. The use of internal controls is not mandatory for use with the QIAsymphony Virus Blood 200 Protocol. If an internal control is not required, Buffer ATE without internal control is added to the samples. Buffer ATE must be placed in slot A of the sample drawer.

 

FAQ ID -2162
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
Yes. The DNA tissue protocols on the QIAsymphony SP purify genomic and mitochondrial DNA.
FAQ ID -2991
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?

The target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony is viral DNA from released as well as cell-associated viruses, co-purified with genomic DNA from blood cells.

 

 

FAQ ID -2158