Is it necessary to adjust the sample DNA concentration of artus® kits?
FAQ ID -1486

In principle, adjustment of DNA concentration prior to the PCR setup is not required. The linear quantitation range of artus® kit systems comprises four to five logarithmic orders of magnitude, as defined by the quantitative standards (QS) series.

Most diagnostic samples lie within this linear range. In HBV or Parvo B19 diagnostics positive material with unusually high viral loads may be observed which result in CT values lower than the highest QS (e.g. QS1 for HBV: 105 IU/μl), indicating that those samples have a virus titer that exceeds the first QS.

Since precise quantitation is not possible outside the linear range of the standard curve, a repetition of the PCR using diluted sample DNA is recommended.