How is the artus® kit sensitivity determined?
FAQ ID -1375

As part of the internal validation the analytical sensitivity is determined by a probit analysis. This is performed by setting up six to eight dilution series (usually in half-logarithmic concentration steps) of the plasmid cloned PCR product starting with the lowest concentration of quantitation standard. Each of these dilutions is run on three different days in eight-fold replicates. From these data the detection limit is calculated using a statistical algorithm; the probit analysis.