Saliva samples were incubated with or without RNAprotect Saliva Reagent at room temperature for 0 or 7 days. After RNA purification, samples were analyzed using Human Genome U133 Plus 2.0 GeneChip Arrays. Comparison between 0 and 7 days shows a 2-fold decrease in signal intensity for 159 transcripts with unstabilized samples, but for only 38 transcripts with RNAprotect stabilized samples (indicated by red spots). (Data kindly provided by Dr. David Wong, University of California, Los Angeles, CA, USA.)
RNAprotect Saliva Reagent stabilized β-actin mRNA levels in saliva for up to 3 days at 25°C. mRNA levels were quantified by real-time RT-PCR using the QuantiTect Probe RT-PCR Kit. Each time point shows the mean CT value from 4 or 6 samples from a saliva pool from 3 donors.
RNAprotect Saliva Reagent stabilized mRNA levels of oral cancer biomarkers for up to 168 hours. Each time point shows the mean CT value from real-time RT-PCR analysis of 3 samples from a saliva pool from 9 donors. H3F3A: H3 histone, family 3A; OAZ1: ornithine decarboxylase antizyme 1; SAT: spermidine/spermine N1-acetyltransferase; S100P: S100 calcium binding protein P; DUSP1: dual specificity phosphatase 1; IL8: interleukin 8; S100A8: S100 calcium binding protein A8 (an abundant mRNA in saliva; not a cancer biomarker). (Data kindly provided by Dr. David Wong, University of California, Los Angeles, CA, USA.)