RNA was isolated from mouse heart using the RNeasy Fibrous Tissue Mini Kit. After reverse transcription using the Omniscript RT Kit, real-time, quantitative, PCR was carried out on the LightCycler System using the QuantiTect SYBR® Green PCR Kit and primers specific for alpha-catenin. Amplification curves are shown for the indicated amounts of total RNA. NTC: no template control.
The RNeasy Fibrous Tissue protocol integrates proteinase K and RNase-free DNase digestion steps during RNA isolation to break down proteins and genomic DNA. This ensures higher yields of high-quality RNA from fiber-rich tissues.
RNA was isolated from the indicated rat tissues using the RNeasy Fibrous Tissue Mini Kit. Real-time, quantitative RT-PCR amplification was carried out with 100 ng total RNA using the QuantiTect Probe RT-PCR Kit and primers and probe specific for alpha-actin.
RNA was isolated from the indicated rat tissues using the RNeasy Fibrous Tissue Mini Kit. RNA quality was determined by [A] formaldehyde agarose gel electrophoresis and [B] scanning with the Agilent 2100 BioAnalyzer.