Lysates from rat lung tissue stabilized in RNAlater were serially diluted to produce samples containing equivalents of 2.5–25 mg tissue. Total RNA was purified from these samples using the QIAcube HT with the RNeasy QIAcube HT Kit and the RNeasy 96 QIAcube HT total RNA Tissue V1.QSP protocol. Purified RNA was eluted in 100 µl RNase-free water. Optical density measurements show a linear relationship between RNA recovery and amount of starting material.
RNA was purified from 5 x 105 Jurkat cells on the QIAcube HT using the RNeasy 96 QIAcube HT Kit with the RNeasy 96 QIAcube HT Total RNA Cell with DNase V1.QSP protocol. Purified RNA was eluted in 110 µl RNase-free water. Quantitative RT-PCR of the human β-actin mRNA was carried out using the QuantiFast Probe RT-PCR Kit with 5 µl eluate in a total volume of 25 µl. The CT values obtained throughout one 96-well plate show even amplification across the whole plate, indicating no significant well-to-well variation in RNA recovery.
RNA was isolated from 10 mg rat tissue (lung, kidney, heart, brain, spleen, and intestine) stabilized in RNAlater RNA Stabilization Reagent. Sample processing was automated on the QIAcube HT, BioRobot Universal System, or the QIAcube using QIAzol Lysis Reagent. A 1 µl aliquot of each purified RNA sample was analyzed on the QIAxcel Advanced using the QIAxcel RNA QC Kit v2.0 and the QX RNA Size Marker 200–6000 nt. The resulting high RNA integrity scores (RIS) indicate recovery of high-quality RNA.
RNA was isolated from 10 mg of various rat tissues stabilized in RNAlater RNA Stabilization Reagent. RNA was purified using QIAzol Lysis Reagent and the QIAcube HT, BioRobot Universal System, or QIAcube. Purified RNA was analyzed by qRT-PCR for pgk1 mRNA using the QuantiFast Probe RT-PCR Kit. CT values indicate similar performance of RNA extracted with the different purification platforms.