DNA was purified from orange juice, chocolate, and peanut butter using the QIAcube HT instrument and the DNeasy mericon 96 QIAcube HT Kit or manually using the DNeasy mericon Food Kit. Purified DNA was analyzed by qPCR of the trnL chloroplast tRNA gene using the QuantiFast SYBR® Green PCR Kit. The resulting CT values indicate similar performance from both purification methods. Diluting the input DNA 1:10 for qPCR analysis shows that all purified DNA was also free of PCR inhibitors.
The DNeasy mericon 96 QIAcube HT Kit enables DNA extraction from food samples in radically shorter time compared to conventional CTAB protocols. Automated purification of 24–96 samples can be carried out on the QIAcube HT instrument in just a few hours with little hands-on time.