PyroMark Q96 Tests

For analyzing gene-specific sequence variation or methylation on PyroMark Q96 ID or PyroMark Q96 MD

Features

  • Detection and quantification of mutations in cancer-related genes
  • Quantification of individual or multiple CpG sites in different genes
  • Estimation of global CpG methylation in defined regions of interest
  • Sequence context provides built-in quality control of the assays
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PyroMark HFE (96)

Cat. No. / ID: 972442

For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
Detection of
HFE
CpG Prader-Willi
CpG MGMT
CpG MLH1
CpG LINE-1
CpG p16
APOE
MTHFR
PyroMark APOE, PyroMark HFE, PyroMark MTHFR, PyroMark Q96 CpG LINE-1, PyroMark Q96 CpG MGMT, PyroMark Q96 CpG MLH1, PyroMark Q96 CpG p16, and PyroMark Q96 CpG Prader-Willi tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

PyroMark Q96 Tests are a range of predesigned assays suitable for highly reliable, quantitative analysis of mutations or SNPs, as well as CpG methylation. Tests designated specifically with 'Q96' are optimized for use with the PyroMark Q96 MD and/or PyroMark Q96 ID Instruments. Other tests, such as PyroMark HFE and PyroMark MTHFR, can additionally be performed on the PyroMark Q24 (with the exception of PyroMark APOE).

Performance

The PyroMark Q96 Tests are highly suitable for accurately measuring the CpG methylation status, as exemplified with the PyroMark Q96 CpG p16 Test (see figure  PyroMark p16 analysis).
See figures

Principle

PyroMark Q96 Tests used in combination with PyroMark Q96 MD and/or PyroMark Q96 ID Systems enable accurate, quantitative mutation (or SNP) and methylation analysis by Pyrosequencing. The assays consist of PCR primers and a sequencing primer.

Procedure

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q96 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.

Applications

PyroMark Q96 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

Supporting data and figures

Resources

Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Scientific Posters (1)

Publications

Y chromosomal STR analysis using Pyrosequencing technology.
Edlund H; Allen M;
Forensic Sci Int Genet; 2009; 3 (2):119-24 2009 Jan 6 PMID:19215881
DNA methylation profiles in monozygotic and dizygotic twins.
Kaminsky ZA; Tang T; Wang SC; Ptak C; Oh GH; Wong AH; Feldcamp LA; Virtanen C; Halfvarson J; Tysk C; McRae AF; Visscher PM; Montgomery GW; Gottesman II; Martin NG; Petronis A;
Nat Genet; 2009; 41 (2):240-5 2009 Jan 18 PMID:19151718
Amelogenin sex determination by pyrosequencing of short PCR products.
Tschentscher F; Frey UH; Bajanowski T;
Int J Legal Med; 2008; 122 (4):333-5 2008 Mar 20 PMID:18351373
Identification of mammal species using species-specific DNA pyrosequencing.
Karlsson AO; Holmlund G;
Forensic Sci Int; 2007; 173 (1):16-20 2007 Feb 28 PMID:17331687
More on contamination: the use of asymmetric molecular behavior to identify authentic ancient human DNA.
Malmström H; Svensson EM; Gilbert MT; Willerslev E; Götherström A; Holmlund G;
Mol Biol Evol; 2007; 24 (4):998-1004 2007 Jan 25 PMID:17255122

FAQ

Can I order the nucleotides from PyroMark Gold Reagents separately?
The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
FAQ ID -2827
Does the PyroMark CpG LINE assay target mouse transposons as well?

The PyroMark CpG LINE-1 assay is specific for human DNA and was not tested on mouse DNA.  Mouse LINE-1 elements differ slightly in sequence and may not be detected.

 

 

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