PyroMark Buffers and Solutions

For optimal preparation and analysis of template DNA by Pyrosequencing

Features

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    PyroMark Denaturation Sol. (500 ml)

    Cat. No. / ID: 979007

    For denaturation of double-stranded PCR product into single-stranded DNA template
    Type
    Denaturation
    Binding
    Annealing
    Wash
    PyroMark Buffers and Solutions are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

    ✓ 24/7 automatic processing of online orders

    ✓ Knowledgeable and professional Product & Technical Support

    ✓ Fast and reliable (re)-ordering

    Product Details

    Generating Pyrosequencing templates from PCR amplicons is fast and straightforward with the PyroMark vacuum workstation. These buffers and solutions are specifically designed for this purpose. PyroMark Binding Buffer facilitates binding of biotinylated PCR amplicons to streptavidin-coated Sepharose beads. These beads are then immobilized with the vacuum tool of the workstation. PyroMark Denaturation Solution gently separates the complementary strand from the biotin-tagged strand destined to become the Pyrosequencing template, and PyroMark Wash Buffer ensures that only the biotin-tagged, single-stranded DNA remains immobilized to the vacuum tool. Finally, when the single-stranded DNA is released into reaction vessels containing the Pyrosequencing primer, hybridization of primer and template is enhanced by PyroMark Annealing Buffer.

    Resources

    Safety Data Sheets (2)
    Download Safety Data Sheets for QIAGEN product components.
    Download Safety Data Sheets for QIAGEN product components.

    FAQ

    Can I order the nucleotides from PyroMark Gold Reagents separately?
    The nucleotides can only be ordered as part of the PyroMark Gold Reagents which also contain enzyme and substrate mix.
    FAQ ID -2827