Plasmid Purification Selection Guides

Simplify plasmid purification kit selection with our helpful selection guides

Not sure which product to choose? Our helpful selection guides make decision making a lot easier! Choose from the following:

Small-scale plasmid purification — 96-well format
Kit name QIAGEN
Plasmid Plus
96 Miniprep

and BioRobot 
Kits
QIAprep 96
Plus Miniprep

and BioRobot
Kits
QIAprep 96
Turbo Miniprep

and BioRobot
Kits
Speed Standard Fast Fast
DNA
grade
Transfection
grade
Transfection
grade
Molecular
biology
grade
Binding
capacity
<50 µg <50 µg <20 µg
Culture
volume
(3–4 x 109
cells/ml)
1.25 –5 ml 1.25 –5 ml 1–5 ml
Yield 15–50 µg 15–50 µg 4–20 µg
Elution
volume
80 µl (100–120
µl for a 5 ml
culture)
80 µl (100–120
µl for a 5 ml
culture)
100 µl
Procedure QIAvac 96,
centrifuge,
BioRobot
Universal
System
QIAvac 96,
centrifuge,
BioRobot
Universal
System
QIAvac 96,
centrifuge,
BioRobot
Universal
System
Time 50 min 40 min 45 min
Small-scale plasmid purification kits
Kit name QIAGEN
Plasmid Mini Kit
QIAprep Spin
Miniprep Kit
Quicklyse
Miniprep Kit
Speed Fast Fast Ultrafast
DNA grade Transfection
grade
Molecular
biology grade
Sequencing
grade
Binding
capacity
<20 µg <50 µg <15 µg
Culture
volume
(3–4 x 109
cells/ml)
3 ml 1–5 ml 1–3 ml
Yield 20 µg 5–50 µg 8–15 µg
Elution volume Variable,
50 µl recommended
50 µl 50 µl
Procedure Centrifuge,
QIAcube Connect
QIAvac 24 Plus,
centrifuge,
QIAcube
Connect
Centrifuge
Time 80 min 30 min 9 min
Large-scale plasmid purification kits
Kit name   EndoFree
Plasmid Kits
QIAGEN
Plasmid Kits
QIAfilter
Plasmid Kits
QIAGEN
Plasmid
Plus Kits
HiSpeed
Plasmid Kits
QIAGEN
Large-Construct
Kit
CompactPrep
Plasmid Kits
Speed Standard Standard Fast
Ultrafast Ultrafast Standard Ultrafast
DNA grade   Endotoxin-free Transfection
grade
Transfection
grade
Transfection
grade
Transfection
grade
Molecular
biology
grade
Molecular
biology
grade
Culture volume
(3–4 x 109
cells/ml)
Midi   _
 25 ml, 100 ml
25 ml,
50–100 ml
20–25 ml,
25–35 ml (hy)
,
50 ml
50 ml,
150 ml
500 ml 25 ml,
50 ml
Maxi 100 ml, 250 ml
 100 ml, 500 ml
100 ml,
250 ml
80–100 ml,
100–130 ml (hy)
,
200 ml
150 ml,
250 ml
 100 ml,
250 ml
Mega 500 ml/1.5 g,
2.5 l / 7.5g
500 ml/1.5 g, 2.5 l/ 7.5g
500 ml/1.5 g,
2.5 l/7.5g
500 ml
500 ml only
rec for hc
Giga 2.5 l/7.5 g,
not rec. for lc
2.5 l/7.5 g, 5 l/15 g
2.5 l/7.5 g,
not rec. for lc
2.5 l
2.5 l only
rec for hc
Kit name   EndoFree
Plasmid Kits
QIAGEN
Plasmid Kits
QIAfilter
Plasmid Kits
QIAGEN
Plasmid
Plus Kits
HiSpeed
Plasmid Kits
QIAGEN
Large-Construct
Kit
CompactPrep
Plasmid Kits
Yield Midi  –
75–100 µg, 20–100 µg
75–100 µg,
20–100 µg
100–200 µg,
150–250 µg (hy)
,
30–100 µg
100–200 µg,
30–100 µg
Up to 50 µg
BAC/PAC/P1 DNA,
up to 200 µg
cosmid DNA 
100–200 µg,
30–100 µg
Maxi 300–500 µg,
50–250 µg
100–500 µg, 20–250 µg
300–500 µg,
50–250 µg
750 µg, 1 mg
(hy)
,
50–250 µg
300–750 µg,
50–250 µg
350–750 µg,
50–250 µg
Mega 1.5–2.5 mg,
0.5–2.5 mg
1.5–2.5 mg, 0.5–2.5 mg
1.5–2.5 mg,
0.5–2.5 mg
Up to 2.5 mg
1.5–2.5 mg,
endotoxin-free
<0.1 EU/µg DNA
Giga 7.5–10 mg
7.5–10 mg, 1–5 mg
7.5–10 mg
Up to 10 mg
7–10 mg,
endotoxin-free
<0.1 EU/µg DNA
Time Midi  –
150 min
110 min
20 min
<45 min
280 min <20 min
Maxi 150 min
160 min
120 min
20 min
<60 min
<20 min
Mega 220 min
220 min
190 min
40 min
50 min
Giga 310 min
320 min
280 min
50 min
50 min
Procedure Lysate clearing
by precipitator
or vacuum,
gravity-flow
Gravity-flow Lysate clearing
by precipitator or
vacuum,
gravity-flow
Vacuum and
centrifuge
Gravity-flow
and precipitator
Centrifuge Centrifuge
and vacuum
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit instructions for use or user operator manual. QIAGEN instructions for use and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services (or your local distributor).
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