When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?

CTAB as a rule increases the density of the aqueous phase. Therefore, MaXtract High Density should be used for the organic extraction of DNA from CTAB samples.

Depending on the amount of CTAB used, the density of the sample can become too high for the MaXtract High Density Tube, leading to a gel barrier above the aqueous phase. In this case, puncture the gel with the tip of a pipette and dilute the aqueous phase with either distilled water or TE buffer. Mix again and then centrifuge. However, we recommend to repeat the separation in a new MaXtract tube.

 

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