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PyroMark PCR Kit

For PCR amplification of template DNA optimized for Pyrosequencing analysis
  • Highly specific amplification of bisulfite converted DNA and gDNA
  • Kit optimized for successful Pyrosequencing analysis
  • High yields of PCR product and no need for optimization
  • Convenient master mix with room-temperature setup
  • Optimized protocols

The PyroMark PCR Kit is specifically optimized for Pyrosequencing analysis, providing highly specific and unbiased amplification of template DNA for various applications, such as mutation detection, SNP analysis, methylation analysis, and sequencing. The kit is provided in a convenient master mix format consisting of HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer for highly specific amplification. In addition, Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC-rich) templates, is provided. The PyroMark PCR Kit is also supplied with CoralLoad Concentrate, which is strongly recommended for use with the PyroMark PCR Master Mix, for highly specific PCR and high yields of amplified DNA. CoralLoad Concentrate contains 2 gel-tracking dyes, allowing PCR products to be loaded directly on a gel and checked prior to Pyrosequencing.

Cat No./ID: 978703
PyroMark PCR Kit (200)
For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
Cat No./ID: 978705
PyroMark PCR Kit (800)
For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
The PyroMark PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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