RNase-Free Water

For use in all molecular biology applications


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    RNase-Free Water

    Cat. No. / ID: 129112

    12 x 1.9 ml RNase-free water prepared without the use of diethylpyrocarbonate (DEPC)
    The RNase-Free Water is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

    Product Details

    RNase-Free Water is pure, quality-tested water suitable for use in all experiments that require RNase-free water, including PCR, RT-PCR, and real-time PCR, and is included in most QIAGEN PCR and RT-PCR Kits, such as the Taq PCR Master Mix Kit, the HotStarTaq Plus Master Mix Kit, and the HotStar HiFidelity Polymerase Kit. RNase-Free Water is prepared with a proprietary process, which yields RNase-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). RNase-Free Water is provided in RNase-free containers.


    Kit Handbooks (1)


    What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
    For best results, all RNA samples should be suspended in RNase-free water. Alternatively, RNase-free 1 mM sodium citrate (pH 6.5) or 10 mM Tris buffer (pH 7.0) may be used. Do not use DEPC-treated water, as most DEPC preparations are contaminated with molecules that are inhibitory to reverse transcription and/or PCR. For long-term storage, RNA preps may be stored at -70 ºC in RNase-free water, or the buffers listed above, or precipitated in ethanol or isopropanol. In order to avoid repeated freeze-thaw cycles, it is recommended that frozen RNA samples be stored as multiple, single-use aliquots.
    FAQ ID -2659