CompactPrep Plasmid Midi Kit
For fast purification of up to 200 µg molecular biology grade plasmid DNA
CompactPrep Plasmid Kits provide fast spin column-based large-scale plasmid purification. Up to 24 samples can be processed in parallel in less than 60 minutes using a vacuum manifold (e.g., QIAvac 24 Plus). Handling of the samples is fast and easy. CompactPrep Plasmid Kits require use of a vacuum device (e.g., QIAvac 24 Plus). Note: CompactPrep Plasmid Kits do not contain QIAfilter Cartridges and require centrifugation for lysate clearing. Buy Products
The CompactPrep Plasmid Midi Kit
is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
CompactPrep Plasmid Kits enable fast, large-scale plasmid purification. The use of a vacuum manifold allows up to 24 samples to be purified in parallel on a single workbench (see figure "Microcentrifuge column format"). Up to 200 µg plasmid DNA is purified in less than 60 minutes from 25 ml (high-copy plasmids) or 50 ml (low-copy plasmids) LB medium. DNA is eluted in 100 µl and concentrations are typically 1–2 µg/µl. CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA with low endotoxin levels (see figure "Low endotoxin levels"). Maximum plasmid yields using LyseBlueLyseBlue reagent is now also included in CompactPrep Kits ensuring high DNA yields. Using a simple visual identification system, LyseBlue reagent prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, cell debris, and genomic DNA. LyseBlue is optional and provided in a separate vial within the kit.
Principle
QIAGEN CompactPrep Plasmid Kits provide a novel method for fast large-scale plasmid preparation. The procedure is based on a nonchaotropic binding chemistry, reducing the total preparation volume to miniprep scale. All protocol steps after cell harvest can be performed at the bench using QIAfilter cartridges, a vacuum manifold, and a standard microcentrifuge. The unique kit chemistry and design of the CompactPrep column ensure large-scale plasmid prep yields using a spin column format.
Procedure
CompactPrep Plasmid Kits provide fast, large-scale, nonchaotropic silica-based plasmid DNA preparation. DNA binding and washing using a spin column on a vacuum manifold (e.g., QIAvac 24 Plus), and DNA elution, using a microcentrifuge, enables the entire plasmid purification process to take place at the bench.
Applications
CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA suitable for use in routine applications, including:
Specifications
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