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DNeasy PowerLyzer Microbial Kit

For the bead-based isolation of high-quality DNA from microbial cultures
  • Short DNA isolation protocol in just 20 minutes from yeast, fungi and bacterial cultures
  • Compatible for use with the PowerLyzer 24 Homogenizer and other bead-based homogenizers
  • Ready-to-use, highly pure DNA for downstream applications

Isolate high-quality genomic DNA from up to 1.8 ml of microbial culture with the DNeasy PowerLyzer Microbial Kit.

 

This kit successfully tests a variety of microorganisms, including Gram (+/-) bacteria, yeast and spores, by lysing microorganisms through a combination of heat, detergent and mechanical 0.1 glass bead digestion. The PowerLyzer 24 Homogenizer is recommended for this kit.

DNeasy PowerLyzer Microbial Kit was formerly sold by MO BIO as PowerLyzer Ultraclean Microbial DNA Isolation Kit.

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Cat No./ID: 12255-50
DNeasy PowerLyzer Microbial Kit (50)
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For the bead-based isolation of high-quality DNA from microbial cultures
Cat No./ID: 12255-S
DNeasy PowerLyzer Microbial Sample (2)
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Isolate high quality DNA from microbial cultures using a bead-based homogenizer.
The DNeasy PowerLyzer Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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Figure 1: The right beads for the right machine.
DNeasy PowerLyzer Microbial Kit (glass beads) and the DNeasy UltraClean Microbial Kit (garnet beads) were used to isolate DNA from gram positive E. faecalis bacteria using the PowerLyzer 24 Homogenizer at 2000 RPM for 5 minutes. Ten µl of each eluate was electrophoresed on a 1% agarose gel. Glass beads in the DNeasy PowerLyzer Microbial kit provided higher DNA yield using the PowerLyzer 24 Homogenizer. The DNeasy UltraClean Microbial Kit uses softer, garnet beads that are recommended for bead beating with a Vortex Genie 2.
Performance


Specifications

Features
Specifications
Bead size 0.1 mm glass
Binding capacity Up to 20 µg per prep
Format Silica Spin Filter Tubes
Processing Bead beating
Sample size 1.8 ml
Sample types Processed cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 20 minutes

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