DNeasy PowerLyzer Microbial Kit

For the bead-based isolation of high-quality DNA from microbial cultures

Short DNA isolation protocol in just 20 minutes from yeast, fungi and bacterial cultures
Compatible for use with the PowerLyzer 24 Homogenizer and other bead-based homogenizers
Ready-to-use, highly pure DNA for downstream applications

Isolate high-quality genomic DNA from up to 1.8 ml of microbial culture with the DNeasy PowerLyzer Microbial Kit.

This kit successfully tests a variety of microorganisms, including Gram (+/-) bacteria, yeast and spores, by lysing microorganisms through a combination of heat, detergent and mechanical 0.1 glass bead digestion. The PowerLyzer 24 Homogenizer is recommended for this kit.

DNeasy PowerLyzer Microbial Kit was formerly sold by MO BIO as PowerLyzer Ultraclean Microbial DNA Isolation Kit.

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Product Details
Specifications
Product Resources

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Cat No./ID: 12255-50

DNeasy PowerLyzer Microbial Kit (50)

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For the bead-based isolation of high-quality DNA from microbial cultures

Cat No./ID: 12255-S

DNeasy PowerLyzer Microbial Sample (2)

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Isolate high quality DNA from microbial cultures using a bead-based homogenizer.

The DNeasy PowerLyzer Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Figure 1: The right beads for the right machine.

DNeasy PowerLyzer Microbial Kit (glass beads) and the DNeasy UltraClean Microbial Kit (garnet beads) were used to isolate DNA from gram positive E. faecalis bacteria using the PowerLyzer 24 Homogenizer at 2000 RPM for 5 minutes. Ten µl of each eluate was electrophoresed on a 1% agarose gel. Glass beads in the DNeasy PowerLyzer Microbial kit provided higher DNA yield using the PowerLyzer 24 Homogenizer. The DNeasy UltraClean Microbial Kit uses softer, garnet beads that are recommended for bead beating with a Vortex Genie 2.

Performance

Specifications

Features	Specifications
Bead size	0.1 mm glass
Binding capacity	Up to 20 µg per prep
Format	Silica Spin Filter Tubes
Processing	Bead beating
Sample size	1.8 ml
Sample types	Processed cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi
Storage temperature	Store at room temperature (15-30°C)
Throughput	1-24 samples
Time per run or per prep	20 minutes

Product Resources

Kit Handbooks (2)

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	MO BIO's PowerLyzer Microbial DNA Kit Handbook	Show details
	DNeasy PowerLyzer Microbial Kit Handbook	Show details

Quick-Start Protocols (1)

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	DNeasy PowerLyzer Microbial Kit QSP	Show details

Safety Data Sheets (1)

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	MSDS DNeasy PowerLyzer Microbial Kit (50)