The figure illustrates the variation in methylation level between 5 different CpG sites in 4 individual tumor samples. Each sample was run in duplicate, and the concordance between the two samples clearly illustrates the reproducibility of Pyrosequencing technology.
Apyrase, a nucleotide-degrading enzyme, continuously degrades unincorporated nucleotides and ATP. When degradation is complete, another nucleotide is added.
Highlighted areas in the Pyrogram trace indicate variable CpG positions (light gray) and built-in bisulfite treatment controls (yellow). The methylation level of each CpG site is indicated in blue boxes on top of the Pyrogram trace. Data kindly provided by Dr. Triantafillos Liloglou, Roy Castle Lung Cancer Foundation, Molecular Oncology, Liverpool.