MagAttract HMW DNA Kit
For isolation of high-molecular-weight genomic DNA
The MagAttract HMW DNA Kit enables purification of high-molecular-weight (100–200 kb) DNA using a simple, magnetic bead-based protocol. The purification procedure gently removes contaminants and inhibitors, while delivering very high yields and purity. The extracted genomic DNA is suitable for numerous applications, including archiving and next-generation sequencing (NGS).
The MagAttract HMW DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Efficient isolation of high-molecular-weight DNA
Isolation of genomic DNA is a crucial step for any DNA analysis method. The key parameters are generally yield, purity, and integrity of the isolated DNA. While for standard methods such as PCR, sequencing, or hybridization techniques, molecular weight in the range of 10–30 kb may be sufficient, some sensitive methods such as the generation of BAC or fosmid libraries or archiving of DNA require higher integrity of genomic DNA.
The MagAttract HMW DNA Kit is specifically designed to deliver high-molecular-weight DNA from a wide range of samples, including human or animal tissue, blood, and bacteria. The kit uses an efficient combination of high-performance magnetic beads and innovative silica-based chemistry. High-performance magnetic particles ensure that the isolated DNA is of high yield and purity.
The convenient MagAttract protocol allows reproducible results in as little as 70 minutes.
Fast and optimized protocol
Optimized buffers and enzymes gently lyse samples, while ensuring minimized fragmentation of genomic DNA. The procedure comprises 4 simple steps: lyse, bind, wash, and elute. Following sample lysis, the DNA binds to the surface of magnetic beads. During the wash steps, contaminants and PCR inhibitors are effectively removed and pure, high-molecular-weight DNA is eluted in Buffer AE (see figure MagAttract HMW DNA Kit procedure).
The high-molecular-weight genomic DNA obtained with the MagAttract HMW DNA Kit is ready for use in NGS experiments. It can be used for the generation of libraries with both small and large inserts and is therefore suitable for all sequencing applications.
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