PAXgene Bone Marrow RNA Kit

The changes in mRNA levels (ΔC_T) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.|Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit. Yields are indicated for 295 samples, from both in-house and external studies. The median yield for the 295 samples was 11.0 μg RNA per 2 ml sample. Note that RNA yields can vary greatly due to the extreme heterogeneity of bone marrow samples.|Bone marrow samples were collected in duplicate and stabilized in PAXgene Bone Marrow RNA Tubes. RNA was purified from the samples using the PAXgene Bone Marrow RNA Kit with no storage or after 5 days storage at 2–8°C. RNA integrity was evaluated using the RNA integrity numbers (RIN), measured on the Agilent 2100 Bioanalyzer. Results are shown for 3 representative donors. The RIN did not change significantly during storage, indicating that the RNA remains stable in the sample.|Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit after the indicated number of freeze–thaw cycles. The RIN values did not change significantly, indicating that RNA remained intact in the samples up to 2 freeze–thaw cycles.|Bone marrow samples were collected from 39 individuals and immediately transferred to [A] PAXgene Bone Marrow RNA Tubes or [B] EDTA tubes. RNA was purified using the PAXgene Bone Marrow RNA Kit or a reference silica-membrane-based method at day 0 or after 2 days storage at room temperature. Expression levels of 5 marker transcripts for each sample (IL-8, p53, cox-2, IL-1β, c-fos) were quantified using real-time RT-PCR. The high correlation of C_T values (threshold cycles) for the samples stabilized in PAXgene Bone Marrow RNA Tubes indicates that the levels of these transcripts did not significantly change during storage, in contrast to the samples stored in EDTA tubes.|Bone marrow samples were stored in PAXgene Bone Marrow RNA Tubes [A] for 3 days at 18–25°C, [B] for 5 days at 2–8°C, or [C] subjected to 2 freeze–thaw cycles. RNA was purified using the PAXgene Bone Marrow RNA Kit, and RNA purity was analyzed by absorbance. The A₂₆₀/A₂₈₀ ratios indicate highly pure RNA from samples stored under different conditions.|