RNeasy Fibrous Tissue Mini Kit

For purification of up to 100 µg total RNA from fiber-rich tissues

Optimized protocols for fibrous tissues
High yields of total RNA
High-quality RNA for all downstream applications
Removal of genomic DNA using DNase I

The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated on the QIAcube Connect. Fibrous tissue samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. For larger samples, the RNeasy Fibrous Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.

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Product		Cat. no.	List price:
RNeasy Fibrous Tissue Mini Kit (50) 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents and Buffers		74704	฿22,528.00	Add to cart

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The RNeasy Fibrous Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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High-quality RNA from fiber-rich tissues.|RNeasy Fibrous Tissue Mini procedure.|Real-time gene expression analysis in a variety of fibrous tissues.|LightCycler analysis of high-quality RNA from heart.|

RNA was isolated from the indicated rat tissues using the RNeasy Fibrous Tissue Mini Kit. RNA quality was determined by [A] formaldehyde agarose gel electrophoresis and [B] scanning with the Agilent 2100 BioAnalyzer.|The RNeasy Fibrous Tissue protocol integrates proteinase K and RNase-free DNase digestion steps during RNA isolation to break down proteins and genomic DNA. This ensures higher yields of high-quality RNA from fiber-rich tissues.|RNA was isolated from the indicated rat tissues using the RNeasy Fibrous Tissue Mini Kit. Real-time, quantitative RT-PCR amplification was carried out with 100 ng total RNA using the QuantiTect Probe RT-PCR Kit and primers and probe specific for alpha-actin.|RNA was isolated from mouse heart using the RNeasy Fibrous Tissue Mini Kit. After reverse transcription using the Omniscript RT Kit, real-time, quantitative, PCR was carried out on the LightCycler System using the QuantiTect SYBR^® Green PCR Kit and primers specific for alpha-catenin. Amplification curves are shown for the indicated amounts of total RNA. NTC: no template control.|

Performance

RNeasy Fibrous Tissue Kits are optimized for use with fiber-rich tissues and the RNeasy Fibrous Tissue procedure ensures easy and efficient isolation of high-quality total RNA (see figure "High-quality RNA from fiber-rich tissues") suitable for any downstream application, including array analysis and real-time RT-PCR (see figures "Real-time gene expression analysis in a variety of fibrous tissues" and "LightCycler analysis of high-quality RNA from heart"). The RNeasy Fibrous Tissue Mini Kit ensures higher yields of RNA from fiber-rich tissue compared with other silica-based methods (see table).

Highest RNA yields using RNeasy Fibrous Tissue Kits compared with other silica-based methods
Rat tissue (10 mg)	RNA yield* (µg) with RNeasy Fibrous Tissue Mini Kit	RNA yield* (µg) with silica kit (Supplier A_V	RNA yield* (µg) with silica kit (Supplier R)	RNA yield* (µg) with silica kit (Supplier S)
Heart	8.2	3.5	<0.5	2.5
Muscle	5.3	0.5	2.0^†	2.2
Skin	6.0	Not determined	Not determined	Not determined
Esophagus	13.9	Not determined	Not determined	Not determined

Principle

RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNeasy Fibrous Tissue Kits are optimized for use with fiber-rich tissues, such as skeletal muscle, heart, and aorta, tissues difficult to lyse due to the abundance of contractile proteins, connective tissue, and collagen. The RNeasy Fibrous Tissue protocol integrates proteinase K and RNase-free DNase digestion steps during RNA isolation to break down proteins and genomic DNA for higher yields of high-quality total RNA. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.

Procedure

Samples of fibrous tissue from 0.5–30 mg are lysed in a guanidine-isothiocyanate buffer. After dilution of the lysate, the sample is treated with proteinase K. Debris is pelleted by centrifugation. Ethanol is then added to the cleared lysate, and RNA is bound to the RNeasy silica membrane. Traces of DNA that may copurify are removed by a DNase treatment on the RNeasy spin column. DNase and any contaminants are washed away, and up to 100 µg high-quality total RNA is eluted in 30–100 µl RNase-free water (see figure "RNeasy Fibrous Tissue Mini procedure").

Applications

RNeasy Fibrous Tissue Kits provide high-quality total RNA from fiber-rich samples that is suitable for any downstream application, including array analysis and real-time RT-PCR.

Brochures & Guides

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	(EN) - Analyzing Gene Expression and Regulation	Show details
	All insights start with the sample: Your comprehensive guide for isolating top-quality RNA	Show details

FAQs

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	How should I quantify RNA isolated with RNeasy Kits? FAQ ID -32	View
	Why does my isolated RNA have a low OD 260/280 ratio? FAQ ID -97	View
	How can I avoid little or no RNA yields when using an RNeasy Kit? FAQ ID -28	View
	Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits? FAQ ID -101	View
	How should RNeasy Kits be stored and how long are they stable? FAQ ID -103	View
	How do you ensure that RNeasy buffers are RNase-free? FAQ ID -113	View
	What is the difference between disruption and homogenization in the RNeasy System? FAQ ID -139	View
	How can I check for purity of RNA isolated using RNeasy Kits? FAQ ID -1023	View
	Which kit should I use for RNA isolation from Cartilage? FAQ ID -1026	View
	What is the difference between Buffers RLT and RLT Plus? FAQ ID -1043	View
	How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set? FAQ ID -1087	View
	Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits? FAQ ID -1164	View
	Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues? FAQ ID -1579	View
	What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit? FAQ ID -1616	View
	Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure? FAQ ID -1619	View
	What is the composition of Buffer RPE? FAQ ID -2797	View
	Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells? FAQ ID -372	View
	Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material? FAQ ID - 3521	View
	What is the smallest sample size that can be used with RNeasy Mini Kits? FAQ ID -485	View
	What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed? FAQ ID -514	View
	Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample? FAQ ID -532	View
	Do you have a kit for RNA isolation from any kind of sample type? FAQ ID -627	View
	I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20	View
	I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745	View
	What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761	View
	Do I need to use RNase inhibitors with the RNeasy Kits? FAQ ID -813	View
	What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy? FAQ ID -942	View
	What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer? FAQ ID -528	View
	What is the composition of Buffer RLT? FAQ ID -2793	View
	What is the maximum binding capacity of RNeasy spin columns? FAQ ID -290	View
	Are RNeasy spin columns sold separately? FAQ ID -159	View
	Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution? FAQ ID -619	View
	What is the composition of Buffer RW1? FAQ ID -2796	View
	How do I safely inactivate biohazardous flow-through material? FAQ ID -12	View
	Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage? FAQ ID -1037	View
	What are the effects of low A260/A230 ratios in RNA preparations on downstream applications? FAQ ID -2248	View
	What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA? FAQ ID -728	View
	How can I check the integrity of RNA purified using RNeasy Kits? FAQ ID -1024	View

Quick-Start Protocols

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	RNeasy Fibrous Tissue Mini Kit (EN)	Show details

Safety Data Sheets

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	Safety Data Sheets Download Safety Data Sheets for QIAGEN product components.	View

Kit Handbooks

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	RNeasy Fibrous Tissue Mini Handbook	Show details

Safety Data Sheets

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	MSDS RNeasy Fibrous Tissue Mini Kit (50)
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