ForenSeq Kintelligence is an integrated solution for forensic genetic genealogy (FGG). Libraries are prepared, sequenced and analyzed in a single, streamlined workflow for efficient assessment of forensically relevant SNPs. Developed according to existing STR-based methods, with input from on-market methods and established research and databases, the kit provides laboratories targeted sequencing and accurate analysis from forensic DNA samples, including those that are low-input and highly degraded. By applying a curated SNP set, a low input requirement, and a forensically validated workflow while building on established methodology, ForenSeq Kintelligence offers the opportunity to advance beyond dead ends and find resolution for cold cases, missing persons and unidentified human remains.
- Sequence compromised samples on a validated system for easy access to results
- Curated content design ensures data privacy with a SNP set selected for forensic relevance
- Keep evidence and associated data in your laboratory and retain full control
- Fully integrated and supported to pair two familiar workflows, ForenSeq and GEDmatch
Cold case samples and missing persons samples are often degraded due to advanced age, exposure to environmental elements and contaminants, and variable extraction and storage procedures. Accordingly, Verogen designed the ForenSeq Kintelligence Kit to deliver high recovery rates from compromised samples, performing studies analyzing degraded and inhibited casework-type samples and conducting a sensitivity assessment with control samples so that the kit reproducibly generates SNP calls across a range of input DNA amounts.
ForenSeq Kintelligence delivered high performance in mock casework studies that generated high call rates from degraded blood samples, teeth samples inhibited by calcium and contemporary bone samples subjected to the insults of cremation, burning and embalming. The sensitivity assessment evaluated control DNA at inputs ranging from 5 ng to 50 pg. The inputs ranging from 5 ng to 250 pg all achieved a call rate of 100%. Performance was similarly high at 100 pg and 50, at 99.9% and 99.6%, respectively (see figure “A sensitivity titration of control DNA across a range of inputs”). High average coverage of approximately 1500× across target sites allows these high call rates and ensures confident calls.
Given exposure to natural elements and prolonged degradation through age, cold case samples are prone to failing quality control checks and yielding poor coverage. This results in low DNA recovery and impacts allele call rates. To maximize recovery, ForenSeq Kintelligence buffers tolerate many common inhibitors including hematin, humic acid, indigo and tannic acid, and microbial contaminants such as E. coli. For 1 ng gDNA samples spiked with 10 ng microbial DNA, Verogen observed a SNP recovery rate of 100%. Control samples inhibited with spike-ins of significant amounts of other common forensic inhibitors demonstrated a high average call rate of 99.2%, comparable with control samples (see figure “An evaluation of inhibitor performance“). The high average call rate enables deep coverage of samples regardless of low input.
||gDNA from hair, bone, teeth, blood, semen and buccal swabs
|DNA input recommendation
||1 ng per sample
|Recommended multiplexing capacity
||3 libraries per run
|Number of SNPs
|Mean amplicon size
||< 150 bp
|Total library prep time
|Hands-on library prep time
||1 hour and 55 minutes
|Total sequencing time