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ipsogen RT Kit

For reverse transcription of total RNA in research use only

Features

  • High-quality reagents for reverse transcription
  • Reliable reagents for reverse transcription
  • Accurate and reproducible reverse transcription

Useful Resources

S_1084_5_GEN_disclaimer

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ipsogen RT Kit (33)

Cat. No. / ID: 679913

For 33 reactions: Reverse transcriptase, 5x RT buffer, dNTP mix, Random primers, RNase Inhibitor, DTT

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The ipsogen RT Kit is intended for research use only and is not for use in diagnostic procedures. The kit provides reagents optimized for reliable and accurate reverse transcription using the Rotor-Gene Q or other thermal cycler instruments. The kit is intended for reverse transcription of total RNA into cDNA for downstream real-time PCR applications.

Principle

The ipsogen RT Kit is a ready-to-use kit for reverse transcription of total RNA into cDNA using reverse transcription-PCR (RT-PCR). The kit provides accurate and reproducible reverse transcription of total RNA into cDNA. Reverse transcriptase is a multifunctional enzyme with 3 distinct enzymatic activities: an RNA-dependent DNA polymerase, a hybrid-dependent exoribonuclease (RNase H), and a DNA-dependent DNA polymerase. In vivo, the combination of these 3 activities allows transcription of the single-stranded RNA genome into double-stranded DNA. The RNA-dependent DNA polymerase activity (reverse transcription) transcribes cDNA from an RNA template. This activity allows synthesis of cDNA for cloning, PCR, and sequencing.

Procedure

The first step is extraction and determination of quality and quantity of total RNA. The second step is reverse transcription of total RNA into cDNA using the ipsogen RT Kit. The use of the ipsogen RT Kit allows reliable and accurate reverse transcription of total RNA. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen RT Kit enables reliable and accurate reverse transcription of total RNA for research use only.

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.