Supplemental Microbial qPCR Mastermix (ROX)

For real-time PCR applications
  • All-in-one mix for PCR using 5' hydrolysis probe-based detection
  • Yields specific products with minimal primer-dimers
  • Enables microbial profiling with instruments that use ROX as a reference dye
Supplemental Microbial qPCR Mastermix (ROX) is highly suited for real-time PCR applications using 5′ hydrolysis probe-based detection that uses ROX as a reference dye. This supplemental mastermix is for use in automated pipetting systems that require more mastermix in addition to the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits. Supplemental Microbial qPCR Mastermix (ROX) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5′ hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.

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Product Cat. no. List price:
Supplemental Microbial qPCR Mastermix (ROX)
2 tubes of 1.35 ml each
330530
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The Supplemental Microbial qPCR Mastermix (ROX) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Principle
Supplemental Microbial qPCR Mastermix (ROX) contains real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate 5′ hydrolysis probe results by preventing the amplification of primer-dimers and other nonspecific products.
Applications
Supplemental Microbial qPCR Mastermix (ROX) is highly suited for use in automated pipetting systems requiring additional mastermix in excess of the mastermix supplied with the Microbial DNA qPCR Arrays and Assay Kits.

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Kit Handbooks
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New version – For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes
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