For the identification of Leptospira DNA
  • A ready-to-use system for real-time PCR
  • Highly sensitive and specific identification of Leptospira DNA
  • Internal Control to monitor PCR inhibition
  • A full license for PCR without additional costs
The cador Leptospira PCR reagent is a highly sensitive and specific assay to identify Leptospira DNA in biological samples.

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cador Leptospira Reagent (96)
For 96 reactions: cador PCR + IC Reagents, cador Leptospira reagents
Effective April 1, 2018, product available exclusively from INDICAL
285815
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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.
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Performance
The cador Leptospira Reagent contains reagents and enzymes for the specific and efficient amplification of highly conserved regions of the Leptospira genome. Inhibition and other malfunctions of PCR are determined by measuring the fluorescence signal in the yellow channel via amplification of the Internal Control (IC), which does not influence the amplification of the analytical PCR for Leptospira. An external positive control (Leptospira Control DNA, containing the targeted Leptospira DNA) is also supplied. Leptospira DNA is detected efficiently and precisely over a wide linear range (see the figure Efficient and precise identification of Leptospira DNA over a wide linear range).
Principle
Pathogen identification by the polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows detection of the accumulating product without having to re-open the reaction tubes afterward, which reduces the risk of cross contamination.
 
The cador Leptospira PCR Reagent contains a primer-probe set specific for a highly conserved region of the Leptospira genome. The reagent also includes a heterologous amplification system as an internal control to ensure the correct interpretation of negative results, and an external positive control. Real-time PCR detection is carried out on a real-time PCR cycler, such as the Rotor-Gene Q.
Procedure
Bacterial DNA can be manually isolated from samples using the QIAamp cador Pathogen Mini Kit. The isolated DNA is ready for use in real-time PCR with the cador Leptospira PCR Reagent on a real-time PCR cycler, such as the Rotor-Gene Q.
Applications
The cador Leptospira PCR Reagent is designed to identify Leptospira DNA in biological samples using polymerase chain reaction (PCR).

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