Qproteome Bacterial Protein Prep Kit
For preparation of soluble bacterial proteins
The kit contains a lysis buffer for preparation of soluble proteins from bacterial cultures for use in proteomics procedures.
The Qproteome Bacterial Protein Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Principle
Bacterial cell walls are disrupted by the combination of a freeze–thaw cycle and a lysis buffer containing lysozyme. The lysis buffer also contains a nuclease, which reduces the viscosity of the lysate and frees proteins associated with nucleic acids. Once lysis is complete, the lysate is centrifuged to precipitate insoluble proteins and cell debris.
Procedure
Lysis buffer is added to frozen cell pellets, cells are resuspended, and incubated on ice for 30 minutes. The lysate is centrifuged to precipitate insoluble proteins and cell debris, and soluble proteins are collected in the supernatant.
Applications
The Qproteome Bacterial Protein Prep Kit is used for preparation of soluble bacterial proteins for proteomics procedures (e.g., 2D-PAGE, mass spectrometry).
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