For rapid and economical automated purification of DNA from a wide range of samples
Complete removal of contamination and inhibitors
Purification of high-quality DNA that is ready for use
Automated procedure saves time and effort
No organic extraction or alcohol precipitation
The BioSprint 96 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 96.
For 384 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
The BioSprint 96 DNA Blood Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Purification of high-quality genomic DNA from mouse tail.
Mouse tail samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 96 DNA Blood Kit with the BioSprint 96 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (2 µl) from 8 out of 96 samples were visualized by agarose gel electrophoresis. M: markers.
Performance
The BioSprint 96 DNA Blood Kit enables purification of DNA from a variety of sample types (see table).
Typical yields from a range of sample types
Sample type
Amount of sample
DNA (µg)
Muscle, bovine
25 mg
13.5 ± 1.5
Heart, bovine
25 mg
3.4 ± 0.6
Spleen, bovine
25 mg
59.1 ± 4.8
Lung, bovine
25 mg
14.7 ± 5.5
Liver, bovine
25 mg
74.0 ± 22.3
Kidney, bovine
25 mg
33.5 ± 5.4
Tail, mouse
1.2 cm (~25 mg)
30.9 ± 4.5
HL-60 cultured cells
2 x 106 cells
9.6 ± 5.6
Blood, human (5–7 x 106 cells per ml)
200 µl
4.5–9.0
Blood, bovine
200 µl
4.4–7.0
Blood, sheep
200 µl
1.2–8.0
Blood, pig
200 µl
4.5–9.0
Blood, dog
200 µl
6.9–16.1
Blood, cat
100 µl
2.9–6.3
Blood, mouse
100 µl
1.5–3.0
Blood, rat
100 µl
1.0–3.0
Dried blood spots, 903 Specimen Collection Paper
1 punch (6 mm [1/4 inch] diameter)
0.10
Dried blood spots, FTA Card
1 punch (6 mm [1/4 inch] diameter)
0.05
Buccal swabs
1 swab
0.8–2.0
Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE.
The BioSprint 96 DNA Blood Kit is used with the BioSprint 96. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 96 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure
Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 96 DNA Blood Kit). We recommend sealing S-Blocks with Tape Pads during sample processing (not needed for rapid blood protocol).
Applications
The BioSprint 96 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:
Southern blotting
PCR and real-time PCR
Genotyping
Features
Specifications
Applications
PCR, real-time PCR, genotyping, blotting
Elution volume
Custom
For automated processing
BioSprint 96 workstation
Format
S-Block, 96-well plate
Main sample type
Blood, tissue, cells
Processing
Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
1 x 25 ml HotStarTaq Master Mix (contains 2500 units HotStarTaq DNA Polymerase PCR Buffer with 3 mM MgCl2, and 400 µM of each dNTP) and 1 x 50 ml RNase-Free Water
For 200 x 20 µl reactions: 4 x 100 µl 7x gDNA Wipeout Buffer, 4 x 50 µl Quantiscript Reverse Transcriptase, 4 x 200 µl 5x Quantiscript RT Buffer, 4 x 50 µl RT Primer Mix, 4 x 1.9 ml RNase-Free Water
