For rapid and economical DNA purification from a wide range of sample types
Complete removal of contamination and inhibitors
Purification of high-quality DNA that is ready for use
Automated procedure saves time and effort
No organic extraction or alcohol precipitation
The BioSprint 15 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 15.
For 360 preps: 5-Rod Covers, 5-Tube Strips, MagAttract Suspension G, Buffers and Reagents
The BioSprint 15 DNA Blood Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
BioSprint DNA procedure.
Reproducible purification of DNA from sheep ear tissue.
Sheep ear tissue samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 15 DNA Blood Kit with the BioSprint 15 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (5 µl) were run on a 0.8% agarose gel in 1x TBE. M: markers (Lambda HindIII).
High PCR performance of DNA purified using the BioSprint 15 DNA Blood Kit.
Human blood was collected and treated with one of 3 anticoagulants: citrate (C), EDTA (E), or heparin (H). DNA was purified from 200 µl blood immediately after blood collection (Fresh) and after one cycle of freezing and thawing (Frozen). DNA was eluted in 200 µl elution buffer. Eluates (1 µl and 10 µl) were subjected to PCR (50 µl reaction volume) using primers to amplify a 1 kb fragment of the Hugl gene. PCR products were run on a 1.5% agarose gel in 1x TBE. M: markers.
Efficient processing of magnetic particles.
The BioSprint instrument transfers magnetic particles from one well or tube to another.
Performance
DNA can be successfully purified from a variety of sample types (see table).
Typical yields from a range of sample types
Sample type
Amount of sample
DNA (µg)
Muscle, bovine
25 mg
16.2 ± 2.5
Heart, bovine
25 mg
5.9 ± 2.6
Spleen, bovine
25 mg
69.1 ± 23.6
Lung, bovine
25 mg
13.8 ± 7.2
Liver, bovine
25 mg
77.8 ± 29.4
Kidney, bovine
25 mg
26.2 ± 18.8
Ear, sheep
30 mg
20.31 ± 1.8
Tail, mouse
1.2 cm (~25 mg)
32.7 ± 4.6
HL-60 cultured cells
2 x 106 cells
10.1 ± 4.7
Blood, human (5–7 x 106 cells per ml)
200 µl
4.5–9.0
Blood, bovine
200 µl
6.2–8.0
Blood, sheep
200 µl
5.6–11.2
Blood, pig
200 µl
4.5–9.0
Blood, dog
200 µl
6.7–12.3
Blood, cat
100 µl
4.4–8.3
Blood, mouse
100 µl
2.0–8.0
Blood, rat
100 µl
1.0–4.0
Blood, bird
10 µl*
15.0 ±6.3
Blood, fish
5 µl*
7.1–10.0
Dried blood spots, 903 Specimen Collection Paper
1 punch (6 mm [1/4 inch] diameter)
0.30
Dried blood spots, FTA Card
1 punch (6 mm [1/4 inch] diameter)
0.12
Buccal swabs
1 swab
0.8–2.0
Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE. * Sample diluted to 200 µl.
The BioSprint 15 DNA Blood Kit is used with the BioSprint 15. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 15 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure
Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 15 DNA Blood Kit).
Applications
The BioSprint 15 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:
Southern blotting
PCR and real-time PCR
Genotyping
Features
Specifications
Applications
PCR, real-time PCR, genotyping, blotting
Elution volume
200 µl
For automated processing
BioSprint 15 workstation
Format
5-tube strip, S-Block
Main sample type
Blood, tissue, cells
Processing
Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water
