Sample to Insight
© QIAGEN 2013–15. All rights reserved

Rotor-Gene Probe RT-PCR Kit

For ultrafast, one-step qRT-PCR using sequence-specific probes on Rotor-Gene cyclers

  • Optimized for ultrafast, reliable results on Rotor-Gene cyclers
  • Sensitive detection of even low copy numbers
  • Accurate detection of a wide range of template amounts
  • Specially formulated, ready-to-use master mix for fast cycling

The Rotor-Gene Probe RT-PCR Kit is designed for use with the Rotor-Gene Q and other Rotor-Gene cyclers, providing ultrafast, highly sensitive quantification of RNA targets with real-time one-step RT-PCR using sequence-specific probes. Outstanding performance is achieved through the combination of a specially optimized master mix and the unique Rotor-Gene cycler. For convenience, the master mix can be stored at 2–8°C.

请登录账号查看购物车
产品 货号 目录价：
Rotor-Gene Probe RT-PCR Kit (400)
For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Probe RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2 x 2 ml RNase-Free Water
 204574
询价
请登录账号查看购物车

Rotor-Gene Probe RT-PCR Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


See trademarks.

  • Main Image Navi
使用序列特异性探针进行灵敏的检测。|Specific primer annealing.|Fast primer annealing.|
10倍稀释的人白细胞RNA（100 ng到1 ng）作为模板进行一步法real-time RT-PCR。使用TaqMan Gene Expression Assay对IL12RB1（白细胞介素-12受体β1）进行分析，重复三次实验。与[B]供应商AII提供的仪器和试剂盒相比，使用[A] Rotor-Gene Q实时荧光定量PCR分析仪和Rotor-Gene Probe RT-PCR Kit灵敏度更高（如较低的CT值）。|Cations in the Rotor-Gene Q PCR buffer increase specific primer annealing. K+ binds to phosphate groups on double-stranded DNA, stabilizing primer annealing. NH4+ destabilizes weak hydrogen bonds between mismatched bases.|[A] Q-Bond in Rotor-Gene Probe RT-PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds. In addition, the unique buffer composition supports the melting of DNA, reducing denaturation and extension times. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing primer annealing time.|
性能
With the Rotor-Gene Probe RT-PCR Kit, low-abundance transcripts are reliably quantified (see figure "Sensitive detection using a sequence-specific probe").
原理

The Rotor-Gene Probe RT-PCR Kit enables reliable quantification of RNA templates on the Rotor-Gene Q without the need for optimization of reaction and cycling conditions. The kit is designed for use with hydrolysis probes (e.g., TaqMan® probes). RNA is used as the template in a reaction where reverse transcription and PCR take place sequentially in the same reaction vessel. Since it is not necessary to transfer the finished RT reaction to another tube for PCR, the real-time RT-PCR procedure is streamlined, making high-throughput analysis possible.

Highly specific amplification is assured through a balanced combination of K+ and NH4+ ions, which promote specific primer annealing, enabling high PCR specificity and sensitivity (see figure "Specific primer annealing"). Fast cycling without compromising performance is achieved using Q-Bond, a novel PCR additive that considerably shortens cycler run times (see figure "Fast primer annealing").

Components of 2x Rotor-Gene Probe RT-PCR Kit*
Component Features Benefits
HotStarTaq Plus DNA Polymerase 5 min activation at 95ºC Set up of qPCR reactions at room temperature
Rotor-Gene Probe RT-PCR Buffer Balanced combination of NH4+ and K+ ions Specific primer annealing ensures reliable qPCR results
Unique Q-Bond additive Faster PCR run times enable faster results and more reactions per day
Rotor-Gene RT Mix   Special blend of reverse transcriptases with a high affinity for RNA     RNA can be transcribed in just 10 minutes, even through complex secondary structures
* Also contains dNTP mix (dATP, dCTP, dGTP, dTTP).
操作流程

The ready-to-use Rotor-Gene Probe RT-PCR Master Mix eliminates the need for optimization of reaction and cycling conditions. Just add template RNA, primers, probe, and the supplied reverse transcriptase mix to the master mix and program the cycler. Instructions are provided in the detailed handbook supplied with the kit.

Hydrolysis probes (e.g., TaqMan® probes) can be used in combination with the Rotor-Gene Probe RT-PCR Kit on the Rotor-Gene Q for fast and sensitive quantification — simply add the primer-probe mix and template to the master mix.

应用

The Rotor-Gene Probe RT-PCR Kit is well suited for use in fast, real-time one-step RT-PCR of RNA targets using sequence specific probes on the Rotor-Gene Q. It is also compatible with Rotor-Gene 3000 and Rotor-Gene 6000 cyclers.

Back to order
特点
参数
应用 Real-time quantification of RNA targets
描述 For ultrafast quantitative real-time one-step RT-PCR using sequence-specific probes
反应类型 Real-time one-step RT-PCR
real-time或终点法PCR Real-time
样本/目标类型 RNA
单一或多重 Single
SYBR Green I或序列特异性探针 Sequence-specific probes
热循环仪 Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000
有/无ROX Without ROX dye

您无权限查看此资源

产品介绍与指南
1
(EN) - Rotor-Gene Q - Pure Detection
Now with even more applications!
显示更多
试剂盒操作手册
1
(EN) - Rotor-gene Probe Handbook
For fast real-time PCR, two-step RT-PCR, and one-step RT-PCR using sequence-specific probes on Rotor-gene cyclers.
显示更多
安全数据表
1
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
浏览
图片
Sensitive detection using sequence-specific probe
使用序列特异性探针进行灵敏的检测。
10倍稀释的人白细胞RNA（100 ng到1 ng）作为模板进行一步法real-time RT-PCR。使用TaqMan Gene Expression Assay对IL12RB1（白细胞介素-12受体β1）进行分析，重复三次实验。与[B]供应商AII提供的仪器和试剂盒相比，使用[A] Rotor-Gene Q实时荧光定量PCR分析仪和Rotor-Gene Probe RT-PCR Kit灵敏度更高（如较低的CT值）。
Specific primer annealing using a balanced combination of K+ and NH4+ ions
Specific primer annealing.
Cations in the Rotor-Gene Q PCR buffer increase specific primer annealing. K+ binds to phosphate groups on double-stranded DNA, stabilizing primer annealing. NH4+ destabilizes weak hydrogen bonds between mismatched bases.
Fast primer annealing with Q-Bond
Fast primer annealing.
[A] Q-Bond in Rotor-Gene Probe RT-PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds. In addition, the unique buffer composition supports the melting of DNA, reducing denaturation and extension times. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing primer annealing time.