Microbial DNA qPCR Multi-Assay Kits

For real-time PCR-based, application-specific microbial identification or profiling; includes 3–6 assay tubes in one box for 20 reactions

Complete kit for microbial profiling with assays, controls, and reagents
Includes a panel of experimentally verified Microbial DNA qPCR Assays
Uses 5' hydrolysis probe detection for high sensitivity and specificity

Microbial DNA qPCR Multi-Assay Kits are a complete solution for real-time PCR-based microbial detection. Microbial DNA qPCR Multi-Assay Kits contain a panel of Microbial DNA qPCR Assays, which are a mix of two PCR primers (10 µM each) and one 5′-hydrolysis probe (5 µM) that enable quantitative real-time PCR. The kit also contains everything required for a successful qPCR run, including Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water and Microbial qPCR Mastermix.

What is the difference between microbial identification and profiling? Identification is determining the microbe’s presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbe’s relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).

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Product	Product no.	Cat. no.	List price:
Microbial DNA Multi-Assay Kit Bartonella henselae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1501Z -3	$666.00	configure
Microbial DNA Multi-Assay Kit Bordetella pertussis Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1502Z -3	$666.00	configure
Microbial DNA Multi-Assay Kit Brucella spp Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1503Z -3	$666.00	configure
Microbial DNA Multi-Assay Kit Campylobacter jejuni Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1504Z -5	$1,241.00	configure
Microbial DNA Multi-Assay Kit Chlamydia trachomatis Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1505Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Clostridium difficile Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1506Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Clostridium perfringens Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1507Z -3	$666.00	configure
Microbial DNA Multi-Assay Kit C. diphtheriae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1508Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Enterococcus faecalis Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1509Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit EHEC Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1510Z -5	$1,241.00	configure
Microbial DNA Multi-Assay Kit Haemophilus influenzae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1511Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Helicobacter pylori Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1512Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Legionella pneumophila Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1513Z -6	$1,527.00	configure
Microbial DNA Multi-Assay Kit Listeria monocytogenes Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1514Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Mycobacterium spp Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1515Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Neisseria meningitidis Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1516Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Pseudomonas aeruginosa Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1517Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Salmonella enterica Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1518Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Shigella dysenteriae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1519Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Strepto. agalactiae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1520Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Strepto. pneumoniae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1521Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Streptococcus pyogenes Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1522Z -4	$953.00	configure
Microbial DNA Multi-Assay Kit Vibrio cholerae Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1523Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Yersinia enterocolitica Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1524Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit Yersinia spp Pathogenicity One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1525Z -7	$1,813.00	configure
Microbial DNA Multi-Assay Kit MRSA: Methicillin-resistant S. aureus One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1526Z -5	$1,241.00	configure
Microbial DNA Multi-Assay Kit Laboratory Rodent Testing One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1551Z -6	$1,527.00	configure
Microbial DNA Multi-Assay Kit SHV Antibiotic Resistance Genes One 20 µl tube for each Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 or 2 tubes Microbial DNA-Free Water (1.35 ml each), 1 or 2 tubes Microbial qPCR Mastermix (1.35 ml each) show details	330043	BBID-1552Z -8	$2,100.00	configure

Microbial DNA qPCR Multi-Assay Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Performance

Lower limit of quantification (LLOQ)

The LLOQ is the lowest concentration of template that still falls into the linear range of the standard curve (see figure, Limit of detection versus lower limit of quantification). Across all Microbial DNA qPCR Assays, 93% have an LLOQ of <100 gene copies (see figure, The LLOQ for all Microbial DNA qPCR Assays reveals high sensitivity). 92% of microbial identification assays meet this LLOQ, as do 95% of virulence factor gene assays and 97% of antibiotic resistance gene assays (see figures, The LLOQ for microbial identification Microbial DNA qPCR Assays reveals high sensitivity, The LLOQ for virulence factor genes Microbial DNA qPCR Assays reveals high sensitivity, and The LLOQ for antibiotic resistance genes Microbial DNA qPCR Assays reveals high sensitivity).

Specificity

Each Microbial DNA qPCR Assay is stringently tested to ensure that it detects only one target species or gene (see figure, Microbial DNA qPCR Assays are highly specific). For assays that do detect more than one target, a list of detected targets and in silico predictions is included on the product sheet.

This specificity is maintained even when samples have high species complexity, such as in stool, sputum, and plaque (see figure, Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples).

Principle

Microbial DNA qPCR Assays are designed to detect bacterial 16S rRNA gene and fungal ribosomal rRNA gene sequences for species identification, as well as detecting virulence factor genes and antibiotic resistance genes using PCR amplification primers and hydrolysis-probe detection.

Procedure

The procedure for Microbial DNA qPCR Multi-Assay Kits is simple, and can be performed in any laboratory with a real-time PCR instrument. DNA is isolated from the sample using the appropriate QIAamp kit, and PCR reactions are set up using the appropriate Microbial qPCR Mastermix for the PCR instrument. Three control reactions are prepared for each sample, including Positive PCR Control, No Template Control, and the Microbial DNA Positive Control, as well as the Microbial DNA qPCR Assays. Real-time PCR is performed and data is analyzed using web-based data analysis software or Excel templates.

Applications

Microbial DNA qPCR Multi-Assay Kits are highly suited for the detection of related bacterial or fungal species, or microbial genes for antibiotic resistance or virulence factors, from a variety of samples.

Brochures & Guides (2)

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	Microbial Product Configurator Microbial product configurator guide	Show details
	Microbial DNA qPCR Arrays For application-specific microbial identification and profiling by real-time PCR	Show details

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	Determination of Inconclusive Results Microbial Identification Data Analysis	Show details
	MRSA Microbial DNA qPCR Multi-Assay Kit Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (1 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (2 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (3 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (4 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (5 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kits (6 + PPC) Microbial Identification Data Analysis	Show details
	Microbial DNA qPCR Multi-Assay Kit Data Analysis Guidelines	Show details
	SHV Microbial DNA qPCR Multi-Assay Kit Microbial Identification Data Analysis	Show details

FAQs (19)

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	What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393	View
	What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394	View
	Which Microbial qPCR Mastermix should I use? FAQ ID — 3395	View
	What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396	View
	What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397	View
	Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398	View
	What sample types can be tested on the arrays/assays? FAQ ID — 3399	View
	Are the assays species-specific? FAQ ID — 3400	View
	What is LLOQ? FAQ ID — 3401	View
	What is the difference between LLOQ and LOD? FAQ ID — 3402	View
	What sequences are used to design the Microbial DNA qPCR Assays? FAQ ID — 3403	View
	Can I measure antibiotic resistance gene expression? FAQ ID — 3404	View
	Can I measure virulence factor gene expression? FAQ ID — 3405	View
	Are the Microbial DNA qPCR Assays wet-lab verified? FAQ ID — 3406	View
	Which probe labels are available for the Microbial DNA qPCR Assays? FAQ ID — 3407	View
	What is the sensitivity for the Microbial DNA qPCR kits? FAQ ID — 3409	View
	How can I calculate the number of bacterial cells that are present in a sample using the Microbial DNA qPCR Assays? FAQ ID — 3411	View
	What species are detected by the Pan Bacteria 1 and Pan Bacteria 3 Assays? FAQ ID - 3427	View
	Is the Microbial qPCR mastermix used in the Microbial DNA assay and in the Microbial DNA arrays free of genomic bacterial DNA? FAQ ID - 3535	View

Scientific Posters (1)

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	Detection and Surveillance of Antibiotic Resistance Genes From Food and Fertilizer Sources Using qPCR Technology	Show details

Technical Information (2)

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	Microbial DNA qPCR Assay/Assay Kits	Show details
	Antibiotic Resistance Gene Resource and Assay List	Show details

Kit Handbooks (1)

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	Microbial DNA qPCR Handbook New version – For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes	Show details

Safety Data Sheets (1)

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	MSDS Microbial DNA qPCR Multi-Assay Kit

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Cat No./ID: 330033

Microbial DNA qPCR Assay Kit

One 20 µl tube of Microbial DNA qPCR Assay, 1 tube Positive PCR Control (20 µl), 1 tube Microbial DNA Positive Control (50 µl), 1 tube Microbial DNA-Free Water (1.35 ml), and 1 tube Microbial qPCR Mastermix (1.35 ml)

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