EZ1 RNA Tissue Mini Kit

For automated purification of high-quality total RNA
  • Purification of up to 30 µg total RNA
  • Reliable processing of up to 10 mg easy-to-lyse tissues
  • RNase-free setup for reliable results
  • Scalable elution volumes
  • Easy protocol setup with credit-card ease of use
The EZ1 RNA Tissue Mini Kit contains all required reagents and labware for automated purification of total RNA from up to 10 mg standard tissue samples using magnetic particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument. The EZ1 RNA Tissue Kit can be used with the EZ1 Advanced XL, the EZ1 Advanced, or the BioRobot EZ1.
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EZ1 RNA Tissue Mini Kit (48)
For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water
 959034
$898.00
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The EZ1 RNA Tissue Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Efficient purification and reproducible yields.|Consistent highly pure RNA.|
Using the EZ1 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A260) with correction for background (A320). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.|RNA was purified from 2-3 replicate tissue samples from each of 4 types of rat tissue using the EZ1 RNA Tissue Mini procedure. Purified total RNA was examined by scanning spectrophotometry. [A] Kidney, 10 mg; [B] liver, 10 mg; [C] pancreas, 5 mg; [D] spleen, 5 mg.|
Performance

The yields and high quality of total RNA purified using the EZ1 RNA Tissue Mini Kit are consistent and reproducible (see figures "Efficient purification and reproducible yields" and "Consistent highly pure RNA").

Principle

Automated purification with the EZ1 RNA Tissue Mini Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card, or the EZ1 Advanced and the EZ1 Advanced RNA Card, or the BioRobot EZ1 and the EZ1 RNA Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.

Procedure

Tissues are first lysed and homogenized in Buffer RLT. RNA is then purified in one step on the instrument following the automated EZ1 RNA procedure. For certain RNA applications that are sensitive to very small amounts of DNA, an integrated DNase digestion step removes genomic DNA contamination. Total RNA is eluted in 200 µl, 100 µl, or 50 µl, ready to use in all downstream applications.

Applications

The high-quality RNA obtained using the EZ1 RNA Tissue Mini Kit is suited for use in many applications, such as:

  • Gene expression analysis using real-time RT-PCR technologies
  • Gene expression analysis using microarray technologies
  • Oncology and pathology research
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Feature
Specifications
Applications PCR, real-time RT-PCR, microarray
Elution volume 50–200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Tissue samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 5–10 µg
Stabilization Yes
Technology Magnetic particles
Time per run or per prep 40 minutes
Yield <30 µg

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Kit Handbooks
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