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ipsogen JAK2 MutaQuant Kit

For quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR
  • Sensitive quantitative assay
  • Accurate quantitation of the JAK2 V617F/G1849T mutation
  • Easy workflow with ready-to-use solutions
The ipsogen JAK2 MutaQuant Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantitation of the JAK2 V617F/G1849T mutation in genomic DNA.
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
0
JAK2 mutation site identification.
1
Accurate detection of JAK2 V617F plasmid standards.
JAK2 V617F plasmid controls (M1-VF to M4-VF), 5 x 101, 5 x 102, 5 x 103, and 5 x 104 copies/5 µl.

2
Reliable detection of JAK2 wild-type plasmid standards.
JAK2 wild-type plasmid controls (WT-1 to WT-4), 5 x 101, 5 x 102, 5 x 103, 5 x 104 copies/5 µl.
Performance
To ensure highest sensitivity, the ipsogen JAK2 MutaQuant Kit has been optimized to detect the JAK2 V617F/G1849T mutation and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of JAK2 V617F plasmid standards and Reliable detection of JAK2 wild-type plasmid standards). The sensitivity has a limit of detection (LOD) of 0.061%. This optimal sensitivity can be obtained on specimens containing at least 10,000 copies of the JAK2 gene (wild-type and V617F mutation).
Principle
The ipsogen JAK2 MutaQuant Kit is a ready-to-use kit for the detection and quantification of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure JAK2 mutation site identification). The kit provides high levels of sensitivity and reproducibility. The ipsogen JAK2 MutaQuant Kit provides 4 plasmid standard dilutions for mutant JAK2 V617F and 4 for the wild-type JAK2 allele. Use of the standards enables accurate quantification of transcripts. Results are expressed as a percent of JAK2 V617F in total JAK2.
Procedure
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaQuant Kit allows detection and quantification of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen JAK2 MutaQuant Kit enables sensitive and reliable detection and quantification of the JAK2 V617F/G1849T mutation for research use only.