QIAamp DNA Blood Maxi Kit

For purification of up to 600 µg genomic, mitochondrial, or viral DNA from blood

  • Rapid purification of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Blood Maxi Kit provides silica-membrane-based DNA purification for labs that process whole blood, plasma, serum, and other body fluids. The QIAamp DNA Blood Maxi Kit is designed for processing 3–10 ml fresh or frozen human whole blood. The QIAamp Maxi spin columns can be easily processed in a centrifuge or on vacuum manifolds (Additional Buffer AW1 [Cat. no. 19081] and Buffer AW2 [Cat. no. 19072] are required for use with the vacuum manifolds).

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产品 货号 目录价:
QIAamp DNA Blood Maxi Kit (10)
For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
51192
询价
QIAamp DNA Blood Maxi Kit (50)
For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
51194
询价

QIAamp DNA Blood Maxi Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


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QIAamp Spin Column实验流程。|储存的血液中的细胞凋亡带型。|长片段PCR。|采用RFLP分析进行亲子鉴定。|
采用优化的缓冲液裂解样本、稳定核酸并增强DNA在QIAamp膜上的选择性吸附。加入乙醇,并将裂解物上样于QIAamp离心柱。使用洗涤缓冲液去除杂质,然后用水或低盐缓冲液洗脱纯的即用型DNA。|将8个血液样本的基因组DNA置于4°C保存1周。使用QIAamp DNA Blood Mini Kit纯化DNA。当血液置于4°C保存时,由于细胞凋亡作用,DNA会迅速降解;在这些样本中可以清晰地观察到形成的凋亡带型。M1:lambda-HindIIIM2:100 bp分子量标准。|从血液中分离的基因组DNA中的人ALDH1基因的10 kb片段的扩增。使用传统方法(酚)或QIAamp DNA Blood Maxi Kit(QIAamp)纯化DNA。直接测序PCR产物。M:1 kb分子量标准。|在三个亲子鉴定案例中,检测使用QIAamp DNA Blood Mini Kit从血液样本中纯化的DNA。1: 母亲;2:孩子;3:疑父;4:孩子+疑父。M:分子量标准。每个泳道上样3 µg DNA。结果:A:被排除的疑父;B & C:确认的疑父。(数据由美国田纳西州纳什维尔Gene Proof Technologies的J. James提供。)|
性能

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure "Apoptotic banding in stored blood"). The purified DNA is suitable for long-range PCR amplification (see figure "Long-range PCR") and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing (see figure "Paternity testing by RFLP analysis").

QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities (see table).

DNA yields from human whole blood with different cell densities.
Leukocytes per mlDNA yield (µg) DNA yield (µg)
2.5 x 105 15.8 95.8
1.0 x 106 60.4 91.5
5.0 x 106 312.0 94.5
1.0 x 107 624.6 94.6
Using the QIAamp DNA Blood Maxi Kit, genomic DNA was purified from 10 ml of human whole blood and eluted in 1 ml elution buffer. The first eluate was loaded onto the column a second time and centrifuged again (i.e., re-eluted). Percentage DNA recovery was calculated by assuming that one leukocyte contains 6.6 pg DNA.

原理
No phenol–chloroform extraction is required. The DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.
操作流程

The QIAamp DNA Blood Maxi Kit simplifies isolation of DNA from blood and related body fluids with a fast spin-column procedure (see flowchart "procedure"). Fresh and frozen whole blood with common anticoagulants, such as EDTA, citrate, or heparin, may be processed. The QIAamp DNA Blood Maxi Kit processes sample sizes of 3–10 ml, with a preparation time of 55 minutes. The typical yield from 10 ml healthy whole blood is 300–600 µg, with an elution volume of 500–2000 µl.

The QIAamp DNA Blood Kits are supplied with a comprehensive handbook containing protocols for DNA isolation from a number of sample sources. QIAamp sample preparation technology is fully licensed.

应用

The QIAamp DNA Blood Maxi Kit provides proven QIAamp technology for purification of DNA from a variatey of materials using Maxi spin columns with 50 ml collection tubes. Sample sources include:

Fresh and frozen whole blood or buffy coat 
Plasma or serum
Bone marrow
Lymphocytes
Body fluids
特点
参数
应用 PCR, blotting
洗脱体积 500–2000 µl
规格 Spin column
主要样本类型 Whole blood, body fluids
处理 Manual (centrifugation or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA, mitochondrial DNA, viral DNA
样本量 3–10 ml
技术 Silica technology
每次运行或制备时间 55 minutes
产量 300–600 µg

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Kit Handbooks
1
For large-scale genomic and viral DNA purification from whole blood, plasma, serum, body fluids, lymphocytes
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参考文献
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图片
QIAamp Spin Column and Vacuum Procedures
QIAamp Spin Column实验流程。
采用优化的缓冲液裂解样本、稳定核酸并增强DNA在QIAamp膜上的选择性吸附。加入乙醇,并将裂解物上样于QIAamp离心柱。使用洗涤缓冲液去除杂质,然后用水或低盐缓冲液洗脱纯的即用型DNA。
Apoptotic banding in stored blood
储存的血液中的细胞凋亡带型。
将8个血液样本的基因组DNA置于4°C保存1周。使用QIAamp DNA Blood Mini Kit纯化DNA。当血液置于4°C保存时,由于细胞凋亡作用,DNA会迅速降解;在这些样本中可以清晰地观察到形成的凋亡带型。M1:lambda-HindIIIM2:100 bp分子量标准。
Efficient long-range PCR
长片段PCR。
从血液中分离的基因组DNA中的人ALDH1基因的10 kb片段的扩增。使用传统方法(酚)或QIAamp DNA Blood Maxi Kit(QIAamp)纯化DNA。直接测序PCR产物。M:1 kb分子量标准。
Paternity Testing by RFLP Analysis
采用RFLP分析进行亲子鉴定。
在三个亲子鉴定案例中,检测使用QIAamp DNA Blood Mini Kit从血液样本中纯化的DNA。1: 母亲;2:孩子;3:疑父;4:孩子+疑父。M:分子量标准。每个泳道上样3 µg DNA。结果:A:被排除的疑父;B & C:确认的疑父。(数据由美国田纳西州纳什维尔Gene Proof Technologies的J. James提供。)