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Bovine Viral Diarrhea Virus (BVDV) (RT-qPCR)
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Manual nucleic acid purification
Automated nucleic acid purification
Real-time RT-PCR
Detection
Step 1: Manual nucleic acid purification
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QIAamp cador Pathogen Mini Kit
For isolation of viral RNA and DNA and bacterial DNA from a variety of animal sample types
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QIAamp Viral RNA Mini Kit
For isolation of viral RNA from cell-free body fluids
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RNeasy Mini Kit
For purification of up to 100 µg total RNA from cells, tissues, and yeast
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virotype Tissue Lysis Reagent
For tissue lysis of cattle ear tissue samples for detection of BVDV RNA using the virotype BVDV RT-PCR Kit
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cador Pathogen 96 QIAcube HT Kit
For automated high-throughput isolation of viral RNA and DNA and bacterial DNA from animal samples
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RNeasy 96 QIAcube HT Kit
For automated high-throughput isolation of RNA from animal and human cells and tissue
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Step 2: Automated nucleic acid purification
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QIAsymphony SP/AS instruments
For fully integrated automation of complete workflows, from sample preparation to assay setup
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QIAcube HT
Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
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Step 3: Real-time RT-PCR
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virotype BVDV RT-PCR Kit
For safe detection of RNA from bovine viral diarrhea virus
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virotype BVDV RT-PCR Reagent
For identification of bovine viral diarrhea virus RNA by real-time RT-PCR
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Step 4: Detection
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Rotor-Gene Q
For outstanding performance in real-time PCR
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